Recombinant HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854)
![Western blot - HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854)](https://www.abcam.com/ps/products/196/ab196854/Images/ab196854-312028-anti-fatty-acid-synthase-antibody-epr7466-hrp-western-blot.jpg "Western blot - HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR7466] to Fatty Acid Synthase
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Overview
-
Product name
HRP Anti-Fatty Acid Synthase antibody [EPR7466]
See all Fatty Acid Synthase primary antibodies -
Description
HRP Rabbit monoclonal [EPR7466] to Fatty Acid Synthase -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Synthetic peptide within Human Fatty Acid Synthase aa 2450 to the C-terminus (C terminal). The exact sequence is proprietary.
-
Positive control
- WB: HeLa, HEK293 and A549 whole cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Dissociation constant (KD)
KD = 1.34 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7466 -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab196854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 273 kDa (predicted molecular weight: 273 kDa). |
Target
-
Function
Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein. -
Tissue specificity
Ubiquitous. Prominent expression in brain, lung, and liver. -
Sequence similarities
Contains 1 acyl carrier domain. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
-
Database links
- Entrez Gene: 2194 Human
- Entrez Gene: 14104 Mouse
- Entrez Gene: 50671 Rat
- Omim: 600212 Human
- SwissProt: P49327 Human
- SwissProt: P19096 Mouse
- SwissProt: P12785 Rat
- Unigene: 83190 Human
see all -
Alternative names
- [Acyl-carrier-protein] S acetyltransferase antibody
- [Acyl-carrier-protein] S malonyltransferase antibody
- 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody
see all
Images
-
Western blot - HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854)All lanes : HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : FASN (Fatty Acid Synthase) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 273 kDa
Observed band size: 260 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteab196854 was shown to specifically react with Fatty Acid Synthase in wild-type HAP1 cells as signal was lost in FASN (Fatty Acid Synthase) knockout cells. Wild-type and FASN (Fatty Acid Synthase) knockout samples were subjected to SDS-PAGE. Ab196854 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
-
![Western blot - HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854)](https://www.abcam.com/ps/products/196/ab196854/Images/ab196854-242078-anti-fatty-acid-synthase-antibody-epr7466-hrp-western-blot.jpg)
Western blot - HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854)All lanes : HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854) at 1/5000 dilution
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 273 kDa
Observed band size: 273 kDa
Exposure time: 8 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196854 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
-
![HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854)](https://www.abcam.com/ps/products/196/ab196854/Images/ab196854-3-benefits-of-recombinant-antibodies.png)
HRP Anti-Fatty Acid Synthase antibody [EPR7466] (ab196854)
References (2)
ab196854 has been referenced in 2 publications.
- Jeong SH et al. Hippo-mediated suppression of IRS2/AKT signaling prevents hepatic steatosis and liver cancer. J Clin Invest 128:1010-1025 (2018). PubMed: 29400692
- Wang Y et al. Cannabidiol attenuates alcohol-induced liver steatosis, metabolic dysregulation, inflammation and neutrophil-mediated injury. Sci Rep 7:12064 (2017). WB . PubMed: 28935932
