Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR10823(B)] to Fibrillarin - Nucleolar Marker
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Product nameHRP Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker
See all Fibrillarin primary antibodies
DescriptionHRP Rabbit monoclonal [EPR10823(B)] to Fibrillarin - Nucleolar Marker
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to residues in Human Fibrillarin was used as an immunogen (UniProt ID: P22087). (The amino acid sequence is considered to be commercially sensitive).
- WB: HEK293, HeLa, HepG2 and MOLT4 whole cell lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab166630)
- Alexa Fluor® 488 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab184817)
- Alexa Fluor® 568 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab202540)
- Alexa Fluor® 594 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab203400)
- Alexa Fluor® 555 Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab203410)
- Anti-Fibrillarin antibody [EPR10823(B)] - BSA and Azide free (ab240147)
Our Abpromise guarantee covers the use of ab196980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).|
FunctionS-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239).
Sequence similaritiesBelongs to the methyltransferase superfamily. Fibrillarin family.
modificationsBy homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).
Cellular localizationNucleus, nucleolus. Fibrillar region of the nucleolus.
- Information by UniProt
- 34 kD nucleolar scleroderma antigen antibody
- 34 kDa nucleolar scleroderma antigen antibody
- Fbl antibody
All lanes : HRP Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (ab196980) at 1/5000 dilution
Lane 1 : HEK293 (Human) Whole Cell Lysate (ab52256)
Lane 2 :
HeLa whole cell lysate (ab150035)
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 8 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab196980 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab196980 has not yet been referenced specifically in any publications.