Key features and details
- HRP Rabbit polyclonal to Fluorescein
- Suitable for: IHC-P, WB, ELISA
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
Product nameHRP Anti-Fluorescein antibody
See all Fluorescein primary antibodies
DescriptionHRP Rabbit polyclonal to Fluorescein
SpecificitySpecific to Fluoresceine.
Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
Species reactivityReacts with: Species independent
Fluorescein coupled to KLH
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA
Concentration information loading...
Purification notesSolid phase hapten antibody capture. Affinity purified and absorbed antibody covalently coupled to a highly purified preparation of Horseradish Peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed. Estimated substitution ratio: HRP:IgG is >1.5
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab19492 in the following tested applications.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/20 - 1/200.|
|ELISA||1/200 - 1/1000.|
RelevanceFluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
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Immunohistochemical analysis of chicken trachea tissue, infected with an avian influenza virus, staining Fluorescein with ab19492.
Fluoroscein-labeled influenza viruses were incubated with tissue overnight at 4°C. Sections were incubated with primary antibody and the signal was amplified with a tyramide signal amplification system.
ab19492 has been referenced in 7 publications.
- Eriksson P et al. Attachment Patterns of Human and Avian Influenza Viruses to Trachea and Colon of 26 Bird Species - Support for the Community Concept. Front Microbiol 10:815 (2019). PubMed: 31057520
- Eriksson P et al. Characterization of avian influenza virus attachment patterns to human and pig tissues. Sci Rep 8:12215 (2018). PubMed: 30111851
- Hasegawa K et al. Somatostatin receptor staining in FFPE sections using a ligand derivative dye as an alternative to immunostaining. PLoS One 12:e0172030 (2017). PubMed: 28182792
- Kraaij MD et al. The NOX2-mediated ROS producing capacity of recipient cells is associated with reduced T cell infiltrate in an experimental model of chronic renal allograft inflammation. Transpl Immunol 30:65-70 (2014). PubMed: 24365514
- Jourdain E et al. The Pattern of Influenza Virus Attachment Varies among Wild Bird Species. PLoS One 6:e24155 (2011). IHC-P . PubMed: 21909418
- Rong YP et al. The BH4 domain of Bcl-2 inhibits ER calcium release and apoptosis by binding the regulatory and coupling domain of the IP3 receptor. Proc Natl Acad Sci U S A 106:14397-402 (2009). IP . PubMed: 19706527
- Mezo M et al. Evaluation of the flukicide treatment policy for dairy cattle in Galicia (NW Spain). Vet Parasitol 157:235-43 (2008). ELISA . PubMed: 18774648