Recombinant HRP Anti-FOXP1 antibody [EPR4113] (ab196978)
![Western blot - HRP Anti-FOXP1 antibody [EPR4113] (ab196978)](https://www.abcam.com/ps/products/196/ab196978/Images/ab196978-242554-anti-foxp1-antibody-epr4113-hrp-western-blot.jpg "Western blot - HRP Anti-FOXP1 antibody [EPR4113] (ab196978)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR4113] to FOXP1
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
-
Product name
HRP Anti-FOXP1 antibody [EPR4113]
See all FOXP1 primary antibodies -
Description
HRP Rabbit monoclonal [EPR4113] to FOXP1 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
-
Immunogen
Synthetic peptide within Human FOXP1 aa 350-450. The exact sequence is proprietary.
-
Positive control
- WB: Jurkat whole cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4113 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab196978 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 95 kDa (predicted molecular weight: 75 kDa). |
Target
-
Function
Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B cell development. -
Involvement in disease
Note=A chromosomal aberration involving FOXP1 is found in acute lymphoblastic leukemia. Translocation t(9;3)(p13;p14.1) with PAX5.
Defects in FOXP1 are the cause of mental retardation with language impairment and autistic features (MRLIAF) [MIM:613670]. It is a developmental disorder characterized by mild to moderate mental retardation, language impairment, and autistic features. Patients show global delay, delayed walking, severely delayed speech development, and behavioral abnormalities, including irritability, hyperactivity, aggression, and stereotypical rigid behaviors. -
Sequence similarities
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain. -
Domain
The leucine-zipper is required for dimerization and transcriptional repression. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 27086 Human
- Entrez Gene: 108655 Mouse
- Entrez Gene: 297480 Rat
- Omim: 605515 Human
- SwissProt: Q9H334 Human
- SwissProt: P58462 Mouse
- SwissProt: Q498D1 Rat
- Unigene: 431498 Human
see all -
Alternative names
- 12CC4 antibody
- FLJ23741 antibody
- Fork head related protein like B antibody
see all
Images
-
Western blot - HRP Anti-FOXP1 antibody [EPR4113] (ab196978)HRP Anti-FOXP1 antibody [EPR4113] (ab196978) at 1/5000 dilution + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 75 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab196978 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
-
![HRP Anti-FOXP1 antibody [EPR4113] (ab196978)](https://www.abcam.com/ps/products/196/ab196978/Images/ab196978-2-benefits-of-recombinant-antibodies.png)
HRP Anti-FOXP1 antibody [EPR4113] (ab196978)
References (2)
ab196978 has been referenced in 2 publications.
- Luo Y et al. A novel epigenetic regulation of circFoxp1 on Foxp1 in colon cancer cells. Cell Death Dis 11:782 (2020). PubMed: 32951006
- Zhou W & Li R microRNA-605 inhibits the oncogenicity of non-small-cell lung cancer by directly targeting Forkhead Box P1. Onco Targets Ther 12:3765-3777 (2019). PubMed: 31190877
