HRP Anti-FOXP3 antibody [mAbcam 22510] (ab22831)

Reviews (2) Submit a question
Western blot - HRP Anti-FOXP3 antibody [mAbcam 22510] (ab22831)

    Key features and details

    • HRP Mouse monoclonal [mAbcam 22510] to FOXP3
    • Suitable for: WB
    • Reacts with: Human
    • Conjugation: HRP
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    Carrier Free Unconjugated

    Overview

    • Product name

      HRP Anti-FOXP3 antibody [mAbcam 22510]
      See all FOXP3 primary antibodies

    • Description

      HRP Mouse monoclonal [mAbcam 22510] to FOXP3

    • Host species

      Mouse

    • Conjugation

      HRP

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Synthetic peptide corresponding to Human FOXP3 aa 400 to the C-terminus.
      (Peptide available as ab16809)

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab22831 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
    Notes
    WB
    1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

    Target

    • Function

      Probable transcription factor. Plays a critical role in the control of immune response.

    • Involvement in disease

      Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.

    • Sequence similarities

      Contains 1 C2H2-type zinc finger.
      Contains 1 fork-head DNA-binding domain.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • AIID antibody
        • DIETER antibody
        • Forkhead box P3 antibody
        • Forkhead box protein P3 antibody
        • FOXP3 antibody
        • FOXP3_HUMAN antibody
        • FOXP3delta7 antibody
        • Immune dysregulation polyendocrinopathy enteropathy X linked antibody
        • Immunodeficiency polyendocrinopathy enteropathy X linked antibody
        • IPEX antibody
        • JM2 antibody
        • MGC141961 antibody
        • MGC141963 antibody
        • OTTHUMP00000025832 antibody
        • OTTHUMP00000025833 antibody
        • OTTHUMP00000226737 antibody
        • PIDX antibody
        • Scurfin antibody
        • XPID antibody
        see all

      Images

      • Western blot - HRP Anti-FOXP3 antibody [mAbcam 22510] (ab22831)
        Western blot - HRP Anti-FOXP3 antibody [mAbcam 22510] (ab22831)
        All lanes : HRP Anti-FOXP3 antibody [mAbcam 22510] (ab22831) at 1/1000 dilution

        Lane 1 : HEK 293 cells overexpressing human FOXP3
        Lane 2 : HEK 293 cells overexpressing human FOXP3 with Human FOXP3 peptide (ab16809) at 1 µg/ml

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 50 kDa
        Observed band size: 50 kDa


        Exposure time: 3 minutes

      References (0)

      Publishing research using ab22831? Please let us know so that we can cite the reference in this datasheet.

      ab22831 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FOXP3 antibody [mAbcam 22510] (HRP)

       Average
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (tonsil)
      Specification
      tonsil
      Fixative
      Formaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
      Permeabilization
      No
      Blocking step
      5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
      Read More

      Mr. Antibody Solutions

      Verified customer

      Submitted Apr 07 2009

      Western blot abreview for Anti-FOXP3 antibody [mAbcam 22510] (HRP)

       Good
      Abreviews
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Human FOXP3 transfected COS cells)
      Specification
      Human FOXP3 transfected COS cells
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 27 2006

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com