Recombinant HRP Anti-G-CSF antibody [EPR3203(N)(B)] (ab199457)
![Western blot - HRP Anti-G-CSF antibody [EPR3203(N)(B)] (ab199457)](https://www.abcam.com/ps/products/199/ab199457/Images/ab199457-245070-anti-g-csf-antibody-epr3203nb-hrp-western-blot.jpg "Western blot - HRP Anti-G-CSF antibody [EPR3203(N)(B)] (ab199457)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR3203(N)(B)] to G-CSF
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-G-CSF antibody [EPR3203(N)(B)]
See all G-CSF primary antibodies -
Description
HRP Rabbit monoclonal [EPR3203(N)(B)] to G-CSF -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human G-CSF aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: P09919 -
Positive control
- WB: K562 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3203(N)(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab199457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
Target
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Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Post-translational
modificationsO-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells). -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 1440 Human
- Entrez Gene: 12985 Mouse
- Entrez Gene: 25610 Rat
- Omim: 138970 Human
- SwissProt: P09919 Human
- SwissProt: P09920 Mouse
- Unigene: 2233 Human
- Unigene: 1238 Mouse
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Alternative names
- C17orf33 antibody
- Colony stimulating factor 3 (granulocyte) antibody
- Colony stimulating factor 3 antibody
see all
Images
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Western blot - HRP Anti-G-CSF antibody [EPR3203(N)(B)] (ab199457)HRP Anti-G-CSF antibody [EPR3203(N)(B)] (ab199457) at 1/5000 dilution + K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate (ab76827) at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab199457 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-G-CSF antibody [EPR3203(N)(B)] (ab199457)](https://www.abcam.com/ps/products/199/ab199457/Images/ab199457-3-benefits-of-recombinant-antibodies.png)
HRP Anti-G-CSF antibody [EPR3203(N)(B)] (ab199457)
References (0)
ab199457 has not yet been referenced specifically in any publications.
