RecombinantRabMAb

Recombinant HRP Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab201813)

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Western blot - HRP Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab201813)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab201813)
  • HRP Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab201813)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR4805] to GABARAP+GABARAPL1+GABARAPL2
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]
    See all GABARAP+GABARAPL1+GABARAPL2 primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR4805] to GABARAP+GABARAPL1+GABARAPL2

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    within Human GABARAP+GABARAPL1+GABARAPL2 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • WB: SHSY-5Y whole cell lysate, human fetal brain tissue. IHC: Human normal testis tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab201813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform antigen retrieval
WB 1/5000. Detects a band of approximately 15 kDa (predicted molecular weight: 14 kDa).

Target

  • Relevance

    GABARAP: Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
    GABARAPL1: Ubiquitin-like modifier that increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Involved in formation of autophagosomal vacuoles. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
    GABARAPL2: Ubiquitin-like modifier involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1 (By similarity). Involved in autophagy. Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.

  • Database links

    Images

    • Western blot - HRP Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab201813)
      Western blot - HRP Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab201813)
      All lanes : HRP Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab201813) at 1/5000 dilution

      Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
      Lane 2 : Brain (Human) Tissue Lysate - fetal normal tissue

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 14 kDa
      Observed band size: 15 kDa
      why is the actual band size different from the predicted?


      Exposure time: 20 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab201813 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab201813)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab201813)

      IHC image of SENP1 staining in a section of formalin-fixed paraffin-embedded human normal testis*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab201813, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • HRP Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab201813)
      HRP Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab201813)

    References (0)

    Publishing research using ab201813? Please let us know so that we can cite the reference in this datasheet.

    ab201813 has not yet been referenced specifically in any publications.

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