Recombinant HRP Anti-GAP43 antibody [EP890Y] (ab196325)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-GAP43 antibody [EP890Y] (ab196325)](https://www.abcam.com/ps/products/196/ab196325/Images/ab196325-238375-IHCAP21876951.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-GAP43 antibody [EP890Y] (ab196325)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP890Y] to GAP43
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-GAP43 antibody [EP890Y]
See all GAP43 primary antibodies -
Description
HRP Rabbit monoclonal [EP890Y] to GAP43 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human GAP43 (C terminal). The exact sequence is proprietary.
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Positive control
- IHC-P: Normal human cerebellum tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP890Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab196325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. -
Sequence similarities
Belongs to the neuromodulin family.
Contains 1 IQ domain. -
Post-translational
modificationsPhosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. -
Cellular localization
Cell membrane. Cell projection > growth cone membrane. Cell junction > synapse. Cytoplasmic surface of growth cone and synaptic plasma membranes. - Information by UniProt
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Database links
- Entrez Gene: 2596 Human
- Entrez Gene: 14432 Mouse
- Entrez Gene: 29423 Rat
- GenBank: NP_002036 Human
- Omim: 162060 Human
- SwissProt: P17677 Human
- SwissProt: P06837 Mouse
- SwissProt: P07936 Rat
see all -
Alternative names
- Axonal membrane protein GAP 43 antibody
- Axonal membrane protein GAP-43 antibody
- B 50 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-GAP43 antibody [EP890Y] (ab196325)
IHC image of GAP43 staining in a section of formalin-fixed paraffin-embedded normal human cerebellum, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab196325 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![HRP Anti-GAP43 antibody [EP890Y] (ab196325)](https://www.abcam.com/ps/products/196/ab196325/Images/ab196325-2-benefits-of-recombinant-antibodies.png)
HRP Anti-GAP43 antibody [EP890Y] (ab196325)
Datasheets and documents
Certificate of Compliance
References (0)
ab196325 has not yet been referenced specifically in any publications.
