Recombinant HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822)
![Western blot - HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822)](https://www.abcam.com/ps/products/201/ab201822/Images/ab201822-246421-anti-gapdh-antibody-epr16891-loading-control-hrp-western-blot.jpg "Western blot - HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR16891] to GAPDH - Loading Control
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-GAPDH antibody [EPR16891] - Loading Control
See all GAPDH primary antibodies -
Description
HRP Rabbit monoclonal [EPR16891] to GAPDH - Loading Control -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Fish, Monkey, Zebrafish, Xenopus tropicalis
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Immunogen
Recombinant fragment within Mouse GAPDH aa 100 to the C-terminus. The exact sequence is proprietary.
Database link: P16858 -
Positive control
- WB: HeLa, NIH3T3, PC-12 whole cell lysates and Human, Mouse, Rat brain tissue lysates. IHC-P: FFPE human kidney renal cell carcinoma tissue sections.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16891 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
- Anti-GAPDH antibody [EPR16891] - BSA and Azide free (ab199553)
- Alexa Fluor® 647 Anti-GAPDH antibody [EPR16891] (ab201272)
- Alexa Fluor® 488 Anti-GAPDH antibody [EPR16891] (ab201768)
- Alexa Fluor® 594 Anti-GAPDH antibody [EPR16891] (ab206371)
- Alexa Fluor® 405 Anti-GAPDH antibody [EPR16891] (ab206372)
- PE Anti-GAPDH antibody [EPR16891] (ab224004)
- FITC Anti-GAPDH antibody [EPR16891] (ab224005)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab201822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). |
Target
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Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. -
Sequence similarities
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. -
Post-translational
modificationsS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated. -
Cellular localization
Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. - Information by UniProt
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Database links
- Entrez Gene: 374193 Chicken
- Entrez Gene: 2597 Human
- Entrez Gene: 100042025 Mouse
- Entrez Gene: 14433 Mouse
- Entrez Gene: 24383 Rat
- Entrez Gene: 685186 Rat
- Entrez Gene: 317743 Zebrafish
- Omim: 138400 Human
see all -
Alternative names
- 38 kDa BFA-dependent ADP-ribosylation substrate antibody
- aging associated gene 9 protein antibody
- Aging-associated gene 9 protein antibody
see all
Images
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Western blot - HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822)All lanes : HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 4 : Human brain tissue lysate - total protein (ab29466)
Lane 5 : Brain (Mouse) Tissue Lysate
Lane 6 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab201822 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822)](https://www.abcam.com/ps/products/201/ab201822/Images/ab201822-254823-anti-gapdh-antibody-epr16891-loading-control-hrp-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822)IHC image of GAPDH staining in a section of formalin-fixed paraffin-embedded human kidney renal cell carcinoma tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab201822 at 1/100 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822)](https://www.abcam.com/ps/products/201/ab201822/Images/ab201822-4-benefits-of-recombinant-antibodies.png)
HRP Anti-GAPDH antibody [EPR16891] - Loading Control (ab201822)
Protocols
References (3)
ab201822 has been referenced in 3 publications.
- Liu H et al. MicroRNA-744 suppresses cell proliferation and invasion of papillary thyroid cancer by directly targeting NOB1. Mol Med Rep 19:1903-1910 (2019). PubMed: 30628685
- Deng Y et al. microRNA-744 is downregulated in glioblastoma and inhibits the aggressive behaviors by directly targeting NOB1. Am J Cancer Res 8:2238-2253 (2018). PubMed: 30555741
- Wang W et al. MicroRNA-592 targets IGF-1R to suppress cellular proliferation, migration and invasion in hepatocellular carcinoma. Oncol Lett 13:3522-3528 (2017). PubMed: 28529580
