Recombinant HRP Anti-GAPDH antibody [EPR6256] - Loading Control (ab185059)
![Western blot - HRP Anti-GAPDH antibody [EPR6256] - Loading Control (ab185059)](https://www.abcam.com/ps/products/185/ab185059/Images/ab185059-218726-anti-gapdh-antibody-epr6256-loading-control-hrp-western-blot.jpg "Western blot - HRP Anti-GAPDH antibody [EPR6256] - Loading Control (ab185059)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR6256] to GAPDH - Loading Control
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-GAPDH antibody [EPR6256] - Loading Control
See all GAPDH primary antibodies -
Description
HRP Rabbit monoclonal [EPR6256] to GAPDH - Loading Control -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human GAPDH aa 250-350. The exact sequence is proprietary.
Database link: P04406 -
Positive control
- WB: 293T, HeLa, HepG2, HUVEC, MCF7 or SH SY5Y cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6256 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915)
- Anti-GAPDH antibody [EPR6256] - BSA and Azide free (ab186930)
- Biotin Anti-GAPDH antibody [EPR6256] - Loading Control (ab195904)
- Alexa Fluor® 647 Anti-GAPDH antibody [EPR6256] (ab215227)
- PerCP Anti-GAPDH antibody [EPR6256] (ab216377)
- APC Anti-GAPDH antibody [EPR6256] (ab221270)
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab185059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa). |
Target
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Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. -
Sequence similarities
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. -
Post-translational
modificationsS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated. -
Cellular localization
Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. - Information by UniProt
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Database links
- Entrez Gene: 2597 Human
- Omim: 138400 Human
- SwissProt: P04406 Human
- Unigene: 544577 Human
- Unigene: 592355 Human
- Unigene: 598320 Human
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Alternative names
- 38 kDa BFA-dependent ADP-ribosylation substrate antibody
- aging associated gene 9 protein antibody
- Aging-associated gene 9 protein antibody
see all
Images
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Western blot - HRP Anti-GAPDH antibody [EPR6256] - Loading Control (ab185059)All lanes : HRP Anti-GAPDH antibody [EPR6256] - Loading Control (ab185059) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Exposure time: 2 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab185059 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406
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![HRP Anti-GAPDH antibody [EPR6256] - Loading Control (ab185059)](https://www.abcam.com/ps/products/185/ab185059/Images/ab185059-2-benefits-of-recombinant-antibodies.png)
HRP Anti-GAPDH antibody [EPR6256] - Loading Control (ab185059)
References (5)
ab185059 has been referenced in 5 publications.
- Huang P et al. Circular RNA cSMARCA5 regulates the progression of cervical cancer by acting as a microRNA-432 sponge. Mol Med Rep 21:1217-1223 (2020). PubMed: 31922210
- Magic Z et al. Ephrin-B2 inhibits cell proliferation and motility in vitro and predicts longer metastasis-free survival in breast cancer. Int J Oncol 55:1275-1286 (2019). PubMed: 31638179
- Elmusrati AA et al. Cancer-associated fibroblasts promote bone invasion in oral squamous cell carcinoma. Br J Cancer 117:867-875 (2017). PubMed: 28742795
- Nagakannan P & Eftekharpour E Differential redox sensitivity of cathepsin B and L holds the key to autophagy-apoptosis interplay after Thioredoxin reductase inhibition in nutritionally stressed SH-SY5Y cells. Free Radic Biol Med 108:819-831 (2017). PubMed: 28478025
- Phuong TTT et al. Calcium influx through TRPV4 channels modulates the adherens contacts between retinal microvascular endothelial cells. J Physiol 595:6869-6885 (2017). PubMed: 28949006
