HRP Anti-GAPDH antibody - Loading Control (ab9385)
Key features and details
- HRP Rabbit polyclonal to GAPDH - Loading Control
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
HRP Anti-GAPDH antibody - Loading Control
See all GAPDH primary antibodies -
Description
HRP Rabbit polyclonal to GAPDH - Loading Control -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Saccharomyces cerevisiae, Xenopus laevis -
Immunogen
Full length native protein (purified) corresponding to Human GAPDH.
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Positive control
- This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; A431; Hek293.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Primary antibody notes
This antibody is the HRP conjugated version of ab9485, for more convenient use as a loading control antibody. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
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Related Products
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (11) |
1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa). Milk blocking may cause 'no bands' problem. Please try BSA as well.
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Notes |
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WB
1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa). Milk blocking may cause 'no bands' problem. Please try BSA as well. |
Target
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Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. -
Sequence similarities
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. -
Post-translational
modificationsS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated. -
Cellular localization
Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. - Information by UniProt
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Database links
- Entrez Gene: 281181 Cow
- Entrez Gene: 2597 Human
- Entrez Gene: 100042025 Mouse
- Entrez Gene: 14433 Mouse
- Entrez Gene: 24383 Rat
- Entrez Gene: 685186 Rat
- Entrez Gene: 380259 Xenopus laevis
- Omim: 138400 Human
see all -
Alternative names
- 38 kDa BFA-dependent ADP-ribosylation substrate antibody
- aging associated gene 9 protein antibody
- Aging-associated gene 9 protein antibody
see all
Images
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All lanes : HRP Anti-GAPDH antibody - Loading Control (ab9385) at 1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteLane 1 - 4 : GAPDH antibody - Loading Control (ab9385) at 1 ug/ml
Lane 1 : HeLa cell lysate at 20 ug
Lane 2 : A431 cell lysate at 20 ug
Lane 3 : Jurkat cell lysate at 20 ug
Lane 4 : 293 cell lysate at 20 ugPerformed under reducing conditions.
Exposure time : 1 minute
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Lanes 1-4 : HRP Anti-GAPDH antibody - Loading Control (ab9385) at 1/5000 dilution ((Blocked in 5% BSA))
Lanes 5-8 : HRP Anti-GAPDH antibody - Loading Control (ab9385) at 1/5000 dilution ((Blocked in 5% MILK))
Lanes 1 & 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)
Lanes 2 & 6 : Jurkat (Human) Whole Cell Lysate (ab52254)
Lanes 3 & 7 :A-431 whole cell lysate (ab7909)
Lanes 4 & 8 :HEK-293 whole cell lysate (ab7902)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
The membrane 1-4 was blocked in 5% BSA (1 hour). The membrane 5-8 was blocked in 5% MILK (1 hour). Abcam routinely uses 5% BSA to block, however following recent customer feedback our labs investigated the effect of 5% milk blocking. We can now confirm that milk is not a suitable blocking agent for this antibody and significantly decreases the signal on the membrane.
Datasheets and documents
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SDS download
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Datasheet download
References (93)
ab9385 has been referenced in 93 publications.
- Wu Q et al. Lnc-hipk1 inhibits mouse adipocyte apoptosis as a sponge of miR-497. Biofactors 48:135-147 (2022). PubMed: 34856026
- Zhang P et al. Pro-inflammatory mediators released by activated monocytes promote aortic valve fibrocalcific activity. Mol Med 28:5 (2022). PubMed: 35062861
- Wang X et al. MicroRNA-125b inhibits the proliferation of vascular smooth muscle cells induced by platelet-derived growth factor BB. Exp Ther Med 22:791 (2021). PubMed: 34093747
- Kiktev DA et al. The fidelity of DNA replication, particularly on GC-rich templates, is reduced by defects of the Fe-S cluster in DNA polymerase d. Nucleic Acids Res 49:5623-5636 (2021). PubMed: 34019669
- Boltengagen M et al. Dynamics of SAS-I mediated H4 K16 acetylation during DNA replication in yeast. PLoS One 16:e0251660 (2021). PubMed: 34014972