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  1. Link

    hrp-gapdh-antibody-mabcam-9484-loading-control-ab9482.pdf

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HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482)

  • Datasheet
  • SDS
Reviews (20)Q&A (3)References (190)

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Western blot - HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482)

    Key features and details

    • HRP Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Human
    • Conjugation: HRP
    • Isotype: IgG2b

    Conjugates logo Related conjugates and formulations

    Carrier Free Unconjugated

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    Overview

    • Product name

      HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control
      See all GAPDH primary antibodies
    • Description

      HRP Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control
    • Host species

      Mouse
    • Conjugation

      HRP
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Rat, Human
      Predicted to work with: Rabbit, Chicken, Cow, Dog, Xenopus laevis, Chinese hamster
    • Immunogen

      Full length native protein (purified) corresponding to Human GAPDH.

    • Positive control

      • WB: HeLa, HEK-293, NIH/3T3 and PC-12 whole cell lysates.
    • General notes

      According to our customer's feedback this antibody does not recognise meningococcal GapA1 or GapA2 (GAPDH) recombinant proteins.

      This antibody has been conjugated with HRP. The concentration of the original antibody was 1 mg/ml, following conjugation the concentration observed will be higher.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
    • Storage buffer

      Preservative: 0.1% 10% Proclin 300 Solution
      Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      IgG fraction
    • Clonality

      Monoclonal
    • Clone number

      mAbcam 9484
    • Myeloma

      Sp2/0-Ag14
    • Isotype

      IgG2b
    • Light chain type

      kappa
    • Research areas

      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Huntington's disease
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Alzheimer's disease
      • Other
      • Isotype/Loading Controls
      • Loading Controls
      • GAPDH
      • Signal Transduction
      • Metabolism
      • Energy Metabolism
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of carbohydrates
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Carbohydrate metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Energy Metabolism
      • Metabolism
      • Types of disease
      • Neurodegenerative disease
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Alternative Versions

      • Anti-GAPDH antibody [mAbcam 9484] - BSA and Azide free (ab174646)
      • Alexa Fluor® 680 Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab184095)
      • Alexa Fluor® 790 Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab184578)
      • Alexa Fluor® 647 Anti-GAPDH antibody [mAbcam 9484] (ab196697)
      • Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)
    • Recombinant Protein

      • Recombinant Mouse GAPDH protein (ab202148)
      • Recombinant Human GAPDH protein (ab77109)
    • Related Products

      • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
      • TMB ELISA Substrate (High Sensitivity) (ab171523)
      • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
      • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
      • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
      • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
      • 450 nm Stop Solution for TMB Substrate (ab171529)
      • 650 nm Stop Solution for TMB Substrate (ab171531)
      • Immunoassay Blocking Buffer (ab171534)
      • Immunoassay Blocking (BSA Free) (ab171535)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab9482 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (20)
    1/1000 - 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 40.2 kDa).

    NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this ab following concerning customer feedback on the lack of signal with some vials. We found that milk significantly decreases signal and is therefore not a suitable blocking agent for this ab (see images of our tests on the datasheet of the unconjugated version, ab9484). This change in characteristics of the ab is due to a recent update to the production process.

    Notes
    WB
    1/1000 - 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 40.2 kDa).

    NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this ab following concerning customer feedback on the lack of signal with some vials. We found that milk significantly decreases signal and is therefore not a suitable blocking agent for this ab (see images of our tests on the datasheet of the unconjugated version, ab9484). This change in characteristics of the ab is due to a recent update to the production process.

    Target

    • Function

      Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
    • Pathway

      Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
    • Sequence similarities

      Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
    • Post-translational
      modifications

      S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
      ISGylated.
    • Cellular localization

      Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
    • Target information above from: UniProt accession P04406 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 374193 Chicken
      • Entrez Gene: 281181 Cow
      • Entrez Gene: 403755 Dog
      • Entrez Gene: 2597 Human
      • Entrez Gene: 100042025 Mouse
      • Entrez Gene: 14433 Mouse
      • Entrez Gene: 100009074 Rabbit
      • Entrez Gene: 24383 Rat
      • Entrez Gene: 685186 Rat
      • Entrez Gene: 380259 Xenopus laevis
      • Omim: 138400 Human
      • SwissProt: P00356 Chicken
      • SwissProt: P10096 Cow
      • SwissProt: Q28259 Dog
      • SwissProt: P04406 Human
      • SwissProt: P16858 Mouse
      • SwissProt: P46406 Rabbit
      • SwissProt: P04797 Rat
      • SwissProt: P51469 Xenopus laevis
      • Unigene: 544577 Human
      • Unigene: 592355 Human
      • Unigene: 598320 Human
      • Unigene: 304088 Mouse
      • Unigene: 309092 Mouse
      • Unigene: 317779 Mouse
      • Unigene: 343110 Mouse
      • Unigene: 392463 Mouse
      • Unigene: 392480 Mouse
      • Unigene: 414470 Mouse
      • Unigene: 458138 Mouse
      • Unigene: 458416 Mouse
      • Unigene: 475698 Mouse
      • Unigene: 129558 Rat
      • Unigene: 91450 Rat
      • Unigene: 995 Xenopus laevis
      see all
    • Alternative names

      • 38 kDa BFA-dependent ADP-ribosylation substrate antibody
      • aging associated gene 9 protein antibody
      • Aging-associated gene 9 protein antibody
      • BARS-38 antibody
      • cb609 antibody
      • EC 1.2.1.12 antibody
      • Epididymis secretory sperm binding protein Li 162eP antibody
      • G3P_HUMAN antibody
      • G3PD antibody
      • G3PDH antibody
      • GAPD antibody
      • GAPDH antibody
      • Glyceraldehyde 3 phosphate dehydrogenase antibody
      • glyceraldehyde 3-PDH antibody
      • Glyceraldehyde-3-phosphate dehydrogenase antibody
      • HEL-S-162eP antibody
      • KNC-NDS6 antibody
      • MGC102544 antibody
      • MGC102546 antibody
      • MGC103190 antibody
      • MGC103191 antibody
      • MGC105239 antibody
      • MGC127711 antibody
      • MGC88685 antibody
      • OCAS, p38 component antibody
      • OCT1 coactivator in S phase, 38-KD component antibody
      • peptidyl cysteine S nitrosylase GAPDH antibody
      • Peptidyl-cysteine S-nitrosylase GAPDH antibody
      • wu:fb33a10 antibody
      see all

    Images

    • Western blot - HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482)
      Western blot - HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482)
      All lanes : HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482) at 1/1000 dilution

      Lane 1 : HeLa Whole Cell Lysate
      Lane 2 : Hek293 Whole Cell Lysate
      Lane 3 : NIH 3T3 Whole Cel lLysate
      Lane 4 : PC12 Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 40.2 kDa
      Observed band size: 37 kDa why is the actual band size different from the predicted?


      Exposure time: 4 minutes

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (190)

    Publishing research using ab9482? Please let us know so that we can cite the reference in this datasheet.

    ab9482 has been referenced in 190 publications.

    • Ding X  et al. Circ_0004771 Accelerates Cell Carcinogenic Phenotypes via Suppressing miR-1253-Mediated DDAH1 Inhibition in Breast Cancer. Cancer Manag Res 13:1-11 (2021). PubMed: 33442289
    • Gavrilovici C  et al. Behavioral Deficits in Mice with Postnatal Disruption of Ndel1 in Forebrain Excitatory Neurons: Implications for Epilepsy and Neuropsychiatric Disorders. Cereb Cortex Commun 2:tgaa096 (2021). PubMed: 33615226
    • Rashed FB  et al. Identification of proteins and cellular pathways targeted by 2-nitroimidazole hypoxic cytotoxins. Redox Biol 41:101905 (2021). PubMed: 33640700
    • Yu Q  et al. Exosomal-mediated transfer of OIP5-AS1 enhanced cell chemoresistance to trastuzumab in breast cancer via up-regulating HMGB3 by sponging miR-381-3p. Open Med (Wars) 16:512-525 (2021). PubMed: 33821219
    • Liang L  et al. Combined Therapy With Polyethylene Glycol-20k and MCC950 Preserves Post-Resuscitated Myocardial Function in a Rat Model of Cardiac Arrest and Cardiopulmonary Resuscitation. J Am Heart Assoc 10:e019177 (2021). PubMed: 33884887
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 20 Abreviews or Q&A

    Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] - Loading Control (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Several cell types, fibroblast, HEK293, LCL)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    Several cell types, fibroblast, HEK293, LCL
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 29 2019

    Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] - Loading Control (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Skeletal muscle)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    20 µg
    Specification
    Skeletal muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 17 2016

    Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] - Loading Control (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    15 µg
    Gel Running Conditions
    Reduced Denaturing (12% polyacrylamide)
    Sample
    Human Cell lysate - whole cell (Huh-7.5 liver cells)
    Specification
    Huh-7.5 liver cells
    Treatment
    siRNA treated cells + untreated control lysate
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 05 2014

    Western blot abreview for Anti-GAPDH [mAbcam 9484] antibody - Loading Control (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (12% gel)
    Sample
    Rat Tissue lysate - other (Cytosolic fractions from skeletal muscles)
    Specification
    Cytosolic fractions from skeletal muscles
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 31 2014

    Western blot abreview for Anti-GAPDH [mAbcam 9484] antibody - Loading Control (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (12% gel)
    Sample
    Mouse Tissue lysate - whole (Skeletal muscle)
    Specification
    Skeletal muscle
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 22 2014

    Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] (HRP) - Loading Control

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Skeletal muscle)
    Loading amount
    20 µg
    Specification
    Skeletal muscle
    Gel Running Conditions
    Reduced Denaturing (12% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. G.K Sakellariou

    Verified customer

    Submitted May 14 2013

    Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] (HRP) - Loading Control

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Heart)
    Loading amount
    50 µg
    Specification
    Heart
    Gel Running Conditions
    Non-reduced Denaturing (8%)
    Blocking step
    BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 30 2012

    Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] (HRP) - Loading Control

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Total Heart)
    Loading amount
    20 µg
    Specification
    Total Heart
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (12% tris glycine)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 18°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 29 2012

    Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] (HRP) - Loading Control

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T)
    Loading amount
    30000 cells
    Specification
    293T
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris-Glycine)
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 10 2011

    Western blot abreview for Anti-GAPDH antibody [mAbcam 9484] (HRP) - Loading Control

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (HeLa cells)
    Loading amount
    500000 cells
    Specification
    HeLa cells
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 12 2010

    1-10 of 20 Abreviews or Q&A

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