HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482)
![Western blot - HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482)](https://www.abcam.com/ps/products/9/ab9482/Images/ab9482-343848-ab9482DS3.jpg "Western blot - HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482)")
Key features and details
- HRP Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
- Isotype: IgG2b
Overview
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Product name
HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control
See all GAPDH primary antibodies -
Description
HRP Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Chicken, Cow, Dog, Xenopus laevis, Chinese hamster
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Immunogen
Full length native protein (purified) corresponding to Human GAPDH.
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Positive control
- WB: HeLa, HEK-293, NIH/3T3 and PC-12 whole cell lysates.
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General notes
According to our customer's feedback this antibody does not recognise meningococcal GapA1 or GapA2 (GAPDH) recombinant proteins.
This antibody has been conjugated with HRP. The concentration of the original antibody was 1 mg/ml, following conjugation the concentration observed will be higher.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
mAbcam 9484 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- Anti-GAPDH antibody [mAbcam 9484] - BSA and Azide free (ab174646)
- Alexa Fluor® 680 Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab184095)
- Alexa Fluor® 790 Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab184578)
- Alexa Fluor® 647 Anti-GAPDH antibody [mAbcam 9484] (ab196697)
- Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)
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Recombinant Protein
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Related Products
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (20) |
1/1000 - 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 40.2 kDa).
NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this ab following concerning customer feedback on the lack of signal with some vials. We found that milk significantly decreases signal and is therefore not a suitable blocking agent for this ab (see images of our tests on the datasheet of the unconjugated version, ab9484). This change in characteristics of the ab is due to a recent update to the production process. |
| Notes |
|---|
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WB
1/1000 - 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 40.2 kDa). NOT SUITABLE for blocking with milk. Block in 5% BSA for 1 hour. Our labs have thoroughly investigated the blocking conditions for this ab following concerning customer feedback on the lack of signal with some vials. We found that milk significantly decreases signal and is therefore not a suitable blocking agent for this ab (see images of our tests on the datasheet of the unconjugated version, ab9484). This change in characteristics of the ab is due to a recent update to the production process. |
Target
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Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. -
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. -
Sequence similarities
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. -
Post-translational
modificationsS-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated. -
Cellular localization
Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. - Information by UniProt
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Database links
- Entrez Gene: 374193 Chicken
- Entrez Gene: 281181 Cow
- Entrez Gene: 403755 Dog
- Entrez Gene: 2597 Human
- Entrez Gene: 100042025 Mouse
- Entrez Gene: 14433 Mouse
- Entrez Gene: 100009074 Rabbit
- Entrez Gene: 24383 Rat
see all -
Alternative names
- 38 kDa BFA-dependent ADP-ribosylation substrate antibody
- aging associated gene 9 protein antibody
- Aging-associated gene 9 protein antibody
see all
Images
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Western blot - HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482)All lanes : HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482) at 1/1000 dilution
Lane 1 : HeLa Whole Cell Lysate
Lane 2 : Hek293 Whole Cell Lysate
Lane 3 : NIH 3T3 Whole Cel lLysate
Lane 4 : PC12 Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40.2 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (158)
ab9482 has been referenced in 158 publications.
- Chen YH et al. MEHP interferes with mitochondrial functions and homeostasis in skeletal muscle cells. Biosci Rep 40:N/A (2020). PubMed: 32255176
- Liu Y et al. CircRNA DONSON contributes to cisplatin resistance in gastric cancer cells by regulating miR-802/BMI1 axis. Cancer Cell Int 20:261 (2020). PubMed: 32581651
- Jan HM et al. Cholesteryl a-D-glucoside 6-acyltransferase enhances the adhesion of Helicobacter pylori to gastric epithelium. Commun Biol 3:120 (2020). PubMed: 32170208
- Sudaryatma PE et al. Bovine Respiratory Syncytial Virus Decreased Pasteurella multocida Adherence by Downregulating the Expression of Intercellular Adhesion Molecule-1 on the Surface of Upper Respiratory Epithelial Cells. Vet Microbiol 246:108748 (2020). PubMed: 32605748
- Yu L et al. LncRNA SNHG16 promotes non-small cell lung cancer development through regulating EphA2 expression by sponging miR-520a-3p. Thorac Cancer 11:603-611 (2020). PubMed: 31953899