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  1. Link

    hrp-gfp-antibody-e385-ab190584.pdf

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Tags & Cell Markers Fusion / Marker Proteins GFP
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RecombinantRabMAb

Recombinant HRP Anti-GFP antibody [E385] (ab190584)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (1) Submit a question References (7)

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Western blot - HRP Anti-GFP antibody [E385] (ab190584)
  • Western blot - HRP Anti-GFP antibody [E385] (ab190584)
  • HRP Anti-GFP antibody [E385] (ab190584)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [E385] to GFP
  • Suitable for: WB
  • Reacts with: Species independent
  • Conjugation: HRP

Conjugates logo Related conjugates and formulations

Carrier Free Unconjugated

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Conjugation
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HRP Conjugation Kit - Lightning-Link® (ab102890)
Primary
Product image
Anti-GFP antibody [E385] (ab32146)
Protein
Product image
Recombinant A. victoria GFP protein (ab84191)

View more associated products

Overview

  • Product name

    HRP Anti-GFP antibody [E385]
    See all GFP primary antibodies
  • Description

    HRP Rabbit monoclonal [E385] to GFP
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK 293 over-expressing GFP lysate and Active. Pure GFP protein, or cells known to overexpress GFP.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin 300 Solution
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    E385
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Fusion / Marker Proteins
    • GFP

Associated products

  • Alternative Versions

    • Anti-GFP antibody [E385] - BSA and Azide free (ab239807)
    • Anti-GFP antibody [E385] (ab32146)
  • Isotype control

    • HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199507)
  • Recombinant Protein

    • Recombinant A. victoria GFP protein (ab84191)
  • Related Products

    • ECL Substrate Kit (High Sensitivity) (ab133406)
    • GFP ELISA Kit (ab171581)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab190584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
Notes
WB
1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).

Target

  • Relevance

    Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.

    Subunit structure: Monomer.

    Tissue specificity: Photocytes.

    Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

    Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.

    Sequence similarities: Belongs to the GFP family.

    Biophysicochemical properties: Absorption: Abs(max)=395 nm
    Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
  • Alternative names

    • GFP antibody
    • Green fluorescent protein antibody

Images

  • Western blot - HRP Anti-GFP antibody [E385] (ab190584)
    Western blot - HRP Anti-GFP antibody [E385] (ab190584)
    All lanes : HRP Anti-GFP antibody [E385] (ab190584) at 1/10000 dilution

    Lane 1 : HEK 293 over-expressing GFP
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 5 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 27 kDa
    Observed band size: 27 kDa


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab190485 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • Western blot - HRP Anti-GFP antibody [E385] (ab190584)
    Western blot - HRP Anti-GFP antibody [E385] (ab190584)
    All lanes : HRP Anti-GFP antibody [E385] (ab190584) at 1/10000 dilution

    Lane 1 : Recombinant A. victoria GFP protein (ab84191)
    Lane 2 : Recombinant RFP protein (ab51993)

    Lysates/proteins at 0.1 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 27 kDa
    Observed band size: 27 kDa


    Exposure time: 10 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab190584 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • HRP Anti-GFP antibody [E385] (ab190584)
    HRP Anti-GFP antibody [E385] (ab190584)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (7)

Publishing research using ab190584? Please let us know so that we can cite the reference in this datasheet.

ab190584 has been referenced in 7 publications.

  • Mueller F  et al. Overlap of NatA and IAP substrates implicates N-terminal acetylation in protein stabilization. Sci Adv 7:N/A (2021). PubMed: 33523899
  • Zhang J  et al. Characterization of GFP-AtPEN1 as a marker protein for extracellular vesicles isolated from Nicotiana benthamiana leaves. Plant Signal Behav 15:1791519 (2020). PubMed: 32657215
  • Singh P  et al. BUB1 and CENP-U, Primed by CDK1, Are the Main PLK1 Kinetochore Receptors in Mitosis. Mol Cell N/A:N/A (2020). PubMed: 33248027
  • Peyret H  et al. Covalent protein display on Hepatitis B core-like particles in plants through the in vivo use of the SpyTag/SpyCatcher system. Sci Rep 10:17095 (2020). PubMed: 33051543
  • Karman Z  et al. Novel perspectives of target-binding by the evolutionarily conserved PP4 phosphatase. Open Biol 10:200343 (2020). PubMed: 33352067
  • Yu X  et al. Legionella effector AnkX interacts with host nuclear protein PLEKHN1. BMC Microbiol 18:5 (2018). PubMed: 29433439
  • Diao Y  et al. A tiling-deletion-based genetic screen for cis-regulatory element identification in mammalian cells. Nat Methods 14:629-635 (2017). WB . PubMed: 28417999

Customer reviews and Q&As

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Western blot abreview for Anti-GFP antibody [E385] (HRP)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Dr. Lin Zhu

Verified customer

Submitted Sep 08 2017

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