Recombinant HRP Anti-GFP antibody [E385] (ab190584)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [E385] to GFP
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-GFP antibody [E385]
See all GFP primary antibodies -
Description
HRP Rabbit monoclonal [E385] to GFP -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK 293 over-expressing GFP lysate and Active. Pure GFP protein, or cells known to overexpress GFP.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E385 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190584 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
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Notes |
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WB
1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). |
Target
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Relevance
Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+ -activated photoprotein aequorin.
Subunit structure: Monomer.
Tissue specificity: Photocytes.
Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.
Sequence similarities: Belongs to the GFP family.
Biophysicochemical properties: Absorption: Abs(max)=395 nm
Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm. -
Alternative names
- GFP antibody
- Green fluorescent protein antibody
Images
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All lanes : HRP Anti-GFP antibody [E385] (ab190584) at 1/10000 dilution
Lane 1 : HEK 293 over-expressing GFP
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 5 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 27 kDa
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab190485 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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All lanes : HRP Anti-GFP antibody [E385] (ab190584) at 1/10000 dilution
Lane 1 :Recombinant A. victoria GFP protein (ab84191)
Lane 2 : Recombinant RFP protein (ab51993)
Lysates/proteins at 0.1 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 27 kDa
Exposure time: 10 secondsThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab190584 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab190584 has been referenced in 8 publications.
- Mueller F et al. Overlap of NatA and IAP substrates implicates N-terminal acetylation in protein stabilization. Sci Adv 7:N/A (2021). PubMed: 33523899
- Jalal AS et al. A CTP-dependent gating mechanism enables ParB spreading on DNA. Elife 10:N/A (2021). PubMed: 34397383
- Zhang J et al. Characterization of GFP-AtPEN1 as a marker protein for extracellular vesicles isolated from Nicotiana benthamiana leaves. Plant Signal Behav 15:1791519 (2020). PubMed: 32657215
- Singh P et al. BUB1 and CENP-U, Primed by CDK1, Are the Main PLK1 Kinetochore Receptors in Mitosis. Mol Cell N/A:N/A (2020). PubMed: 33248027
- Peyret H et al. Covalent protein display on Hepatitis B core-like particles in plants through the in vivo use of the SpyTag/SpyCatcher system. Sci Rep 10:17095 (2020). PubMed: 33051543
- Karman Z et al. Novel perspectives of target-binding by the evolutionarily conserved PP4 phosphatase. Open Biol 10:200343 (2020). PubMed: 33352067
- Yu X et al. Legionella effector AnkX interacts with host nuclear protein PLEKHN1. BMC Microbiol 18:5 (2018). PubMed: 29433439
- Diao Y et al. A tiling-deletion-based genetic screen for cis-regulatory element identification in mammalian cells. Nat Methods 14:629-635 (2017). WB . PubMed: 28417999