Recombinant HRP Anti-Glutaminase antibody [EP7212] (ab200408)
![Western blot - HRP Anti-Glutaminase antibody [EP7212] (ab200408)](https://www.abcam.com/ps/products/200/ab200408/Images/ab200408-311992-anti-glutaminase-antibody-ep7212-hrp-western-blot.jpg "Western blot - HRP Anti-Glutaminase antibody [EP7212] (ab200408)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP7212] to Glutaminase
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-Glutaminase antibody [EP7212]
See all Glutaminase primary antibodies -
Description
HRP Rabbit monoclonal [EP7212] to Glutaminase -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Glutaminase aa 50-150. The exact sequence is proprietary.
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Positive control
- WB: Human fetal kidney lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP7212 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Glutaminase antibody [EP7212] (ab156876)
- Alexa Fluor® 488 Anti-Glutaminase antibody [EP7212] (ab200406)
- Alexa Fluor® 647 Anti-Glutaminase antibody [EP7212] (ab200407)
- Alexa Fluor® 555 Anti-Glutaminase antibody [EP7212] (ab207272)
- Anti-Glutaminase antibody [EP7212] - BSA and Azide free (ab214802)
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab200408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa). |
Target
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Function
Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. -
Tissue specificity
KGA is expressed predominantly in brain and kidney but not in liver, isoform 3 is expressed principally in cardiac muscle and pancreas but not in liver or brain, and isoform 2 is expressed solely in cardiac and skeletal muscle. -
Sequence similarities
Belongs to the glutaminase family.
Contains 1 ANK repeat. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 2744 Human
- Omim: 138280 Human
- SwissProt: O94925 Human
- Unigene: 116448 Human
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Alternative names
- AAD20 antibody
- DKFZp686O15119 antibody
- FLJ10358 antibody
see all
Images
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Western blot - HRP Anti-Glutaminase antibody [EP7212] (ab200408)All lanes : HRP Anti-Glutaminase antibody [EP7212] (ab200408) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : GLS (Glutaminase) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 73 kDa
Exposure time: 12 minutesab200408 was shown to recognize Glutaminase in wild-type HAP1 cells as signal was lost at the expected MW in GLS (Glutaminase) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GLS (Glutaminase) knockout samples were subjected to SDS-PAGE. Ab200408 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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![Western blot - HRP Anti-Glutaminase antibody [EP7212] (ab200408)](https://www.abcam.com/ps/products/200/ab200408/Images/ab200408-246674-anti-glutaminase-antibody-ep7212-hrp-western-blot.jpg)
Western blot - HRP Anti-Glutaminase antibody [EP7212] (ab200408)HRP Anti-Glutaminase antibody [EP7212] (ab200408) at 1/5000 dilution + Kidney (Human) Tissue Lysate - fetal normal tissue at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 73 kDa
Observed band size: 73 kDa
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab200408 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-Glutaminase antibody [EP7212] (ab200408)](https://www.abcam.com/ps/products/200/ab200408/Images/ab200408-4-benefits-of-recombinant-antibodies.png)
HRP Anti-Glutaminase antibody [EP7212] (ab200408)
References (1)
ab200408 has been referenced in 1 publication.
- Karaca M et al. Liver Glutamate Dehydrogenase Controls Whole Body Energy Partitioning Through Amino Acid-Derived Gluconeogenesis and Ammonia Homeostasis. Diabetes N/A:N/A (2018). PubMed: 30002133
