Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR13022(B)] to Glutamine Synthetase
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Product nameHRP Anti-Glutamine Synthetase antibody [EPR13022(B)]
See all Glutamine Synthetase primary antibodies
DescriptionHRP Rabbit monoclonal [EPR13022(B)] to Glutamine Synthetase
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human Glutamine Synthetase aa 250-350 (Cysteine residue). The exact sequence is proprietary.
Database link: P15104
- WB: Human fetal liver lysate.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab199198 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2500. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).|
FunctionThis enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
Involvement in diseaseDefects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
Sequence similaritiesBelongs to the glutamine synthetase family.
Developmental stageExpressed during early fetal stages.
Cellular localizationCytoplasm. Mitochondrion.
- Information by UniProt
- cell proliferation-inducing protein 59 antibody
- Cgl2214 antibody
- GLNA antibody
HRP Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab199198) at 1/2500 dilution + Liver (Human) Tissue Lysate - fetal normal tissue at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with 199198 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab199198 has not yet been referenced specifically in any publications.