Validated using a knockout cell lineRecombinantRabMAb

Recombinant HRP Anti-GSK3 beta antibody [Y174] (ab196911)

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Western blot - HRP Anti-GSK3 beta antibody [Y174] (ab196911)
  • Western blot - HRP Anti-GSK3 beta antibody [Y174] (ab196911)
  • HRP Anti-GSK3 beta antibody [Y174] (ab196911)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [Y174] to GSK3 beta
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-GSK3 beta antibody [Y174]
    See all GSK3 beta primary antibodies

  • Description

    HRP Rabbit monoclonal [Y174] to GSK3 beta

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide within Human GSK3 beta aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: A431 whole cell lysate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Applications

Our Abpromise guarantee covers the use of ab196911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 46 kDa).

Target

  • Function

    Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair.

  • Tissue specificity

    Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.

  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Phosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216.

  • Cellular localization

    Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Glycogen Synthase Kinase 3 Beta antibody
      • Glycogen synthase kinase-3 beta antibody
      • GSK 3 beta antibody
      • GSK-3 beta antibody
      • GSK3B antibody
      • GSK3B_HUMAN antibody
      • GSK3beta isoform antibody
      • Serine/threonine-protein kinase GSK3B antibody
      see all

    Images

    • Western blot - HRP Anti-GSK3 beta antibody [Y174] (ab196911)
      Western blot - HRP Anti-GSK3 beta antibody [Y174] (ab196911)
      All lanes : HRP Anti-GSK3 beta antibody [Y174] (ab196911) at 1/5000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : GSK3B knockout HAP1 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 46 kDa
      Observed band size: 46 kDa


      Exposure time: 20 minutes


      ab196911 was shown to recognize GSK3 beta in wild-type HAP1 cells as signal was lost at the expected MW in GSK3B knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GSK3B knockout samples were subjected to SDS-PAGE. Ab196911 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

    • Western blot - HRP Anti-GSK3 beta antibody [Y174] (ab196911)
      Western blot - HRP Anti-GSK3 beta antibody [Y174] (ab196911)
      HRP Anti-GSK3 beta antibody [Y174] (ab196911) at 1/5000 dilution + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 46 kDa
      Observed band size: 38 kDa
      why is the actual band size different from the predicted?


      Exposure time: 2 seconds


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196911 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • HRP Anti-GSK3 beta antibody [Y174] (ab196911)
      HRP Anti-GSK3 beta antibody [Y174] (ab196911)

    References (0)

    Publishing research using ab196911? Please let us know so that we can cite the reference in this datasheet.

    ab196911 has not yet been referenced specifically in any publications.

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