HRP Anti-GST antibody (ab3416)

Reviews (3)Q&A (8)References (25)
ELISA - HRP Anti-GST antibody (ab3416)
  • ELISA - HRP Anti-GST antibody (ab3416)

Key features and details

  • HRP Rabbit polyclonal to GST
  • Suitable for: WB, ELISA
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgG

Overview

  • Product name

    HRP Anti-GST antibody
    See all GST primary antibodies

  • Description

    HRP Rabbit polyclonal to GST

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WB, ELISAmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Full length native protein (purified) corresponding to Schistosoma japonicum GST. Glutathione-S-transferase (GST) from Schistosoma japonicum.

  • General notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/5000.
ELISA (2)
1/10000 - 1/25000.
Notes
WB
1/1000 - 1/5000.
ELISA
1/10000 - 1/25000.

Target

  • Relevance

    GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.

  • Alternative names

    • Glutathione S Transferase antibody
    • Glutathione S-transferase class-mu 26 kDa isozyme antibody
    • GST antibody
    • Sj26 antigen antibody
    • SjGST antibody
    see all

Images

  • ELISA - HRP Anti-GST antibody (ab3416)
    ELISA - HRP Anti-GST antibody (ab3416)This image is courtesy of an anonymous abreview.

    Graph of ELISA measuring levels of bound GST-tagged mosquito proteins. 5g of target protein was coated onto an ELISA plate and incubated overnight at 4°C. The plate was washed and GST-tagged mosquito proteins were incubated for an hour at RT. The proteins were washed off, GST-HRP antibody (ab3416) was diluted 1/5000 in 1%BSA in PBS and added for 30 min at RT. The plate was washed and TMB substrate was added for 20 min at RT. Stop solution was added and the O.D. of the wells read at 450 nm.

    See Abreview

  • ELISA - HRP Anti-GST antibody (ab3416)
    ELISA - HRP Anti-GST antibody (ab3416)This image is courtesy of an anonymous abreview.

    Graph of ELISA measuring levels of bound GST-tagged mosquito proteins. 5g of target protein was coated onto an ELISA plate and incubated overnight at 4°C. The plate was washed and GST-tagged mosquito proteins were incubated for an hour at RT. The proteins were washed off, GST-HRP antibody (ab3416) was diluted 1/5000 in 1%BSA in PBS and added for 30 min at RT. The plate was washed and TMB substrate was added for 20 min at RT. Stop solution was added and the O.D. of the wells read at 450 nm.

    See Abreview

References (25)

Publishing research using ab3416? Please let us know so that we can cite the reference in this datasheet.

ab3416 has been referenced in 25 publications.

  • Moreno R  et al. The stress-responsive kinase DYRK2 activates heat shock factor 1 promoting resistance to proteotoxic stress. Cell Death Differ 28:1563-1578 (2021). PubMed: 33268814
  • Zyner KG  et al. Genetic interactions of G-quadruplexes in humans. Elife 8:N/A (2019). PubMed: 31287417
  • Liu K  et al. PI31 Is an Adaptor Protein for Proteasome Transport in Axons and Required for Synaptic Development. Dev Cell 50:509-524.e10 (2019). PubMed: 31327739
  • Liu H  et al. Surface Loops in a Single SH2 Domain Are Capable of Encoding the Spectrum of Specificity of the SH2 Family. Mol Cell Proteomics 18:372-382 (2019). PubMed: 30482845
  • Misra R  et al. Tuning the Mycobacterium tuberculosis Alternative Sigma Factor SigF through the Multidomain Regulator Rv1364c and Osmosensory Kinase Protein Kinase D. J Bacteriol 201:N/A (2019). PubMed: 30642988
View all Publications for this product

Customer reviews and Q&As

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1-10 of 11 Abreviews or Q&A

ELISA abreview for Anti-GST antibody (HRP)

 Excellent
Abreviews
abreview image
Application
ELISA
Sample
Anopheles stephensi Recombinant protein (GST-tagged mosquito protein made in e coli)
Specification
GST-tagged mosquito protein made in e coli
Type
Direct
Read More

Abcam user community

Verified customer

Submitted Dec 08 2014

ELISA abreview for Anti-GST antibody (HRP)

 Good
Abreviews
abreview image
Application
ELISA
Sample
Aedes aegypti (Mosquito) Recombinant protein (GST-tagged mosquito protein made in e coli)
Specification
GST-tagged mosquito protein made in e coli
Type
Direct
Read More

Abcam user community

Verified customer

Submitted Dec 08 2014

Western blot abreview for Anti-GST antibody (HRP)

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
1 µg
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Sample
Aedes aegypti (Mosquito) Recombinant protein (Aedes salivary protein)
Specification
Aedes salivary protein
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Nov 26 2014

Question

Thanks you very much! I appreciate the compensation while this wasn’t necessary. We’ll have a look at the new batch and compare it with the old one.

The address didn’t change:

Onderwijsboulevard 219

5223DE Den Bosch

The Netherlands

Thanks again!

Read More
Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1241474.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

PS: The shipment will be direct to you.

Read More

Question

We double checked our constructs. On the left some his/thrombin tagged proteins stained with anti-HIS. On the right the same blot stained with your anti GST (stripped first)

These proteins contain a thrombin site but no GST! Are you 100% sure you used GST isolated from Schistosoma japonicum as antigen?? It’s much easier/cheaper to express this protein recombinant in e.coli and then inject it in mice. But if you do it like that, often it contains a thrombin site because that’s introduced by the plasmid suppliers

Please let me know what you think.

Thanks

Kind regards,

Read More
Answer

Thank very much for your patience as it took us more than a month to test this antibody.

The collaborator laboratory have confirmed that they have immunized rabbits with GST isolated from Schistosoma japonicum. The following is their response

"we tested the antibody (by ELISA) versus various 6HIS preps and have found very little, if any reaction to 6HIS. Despite this, we absorbed the antibody versus various 6HIS preps that we have coupled to gels and then retested hoping to see any kind of change. Unfortunately, no change was observed by ELISA...this doesn't mean that there wouldn't be a change in other kinds of testing but for how we can test we are not unable to see a difference before or after absorbing.

They have also confirmed that the antibody does not have any relation to thrombin protein so it should be binding.

I am sorry we are unable to determine what could have gone wrong with this product. Our test show antibody is specific to the target, I guess the way left is trying a different lot. So if you are interested I can send you a new lot free of charge or another antibody against the same target.

I hope this information will be nevertheless helpful. Please do let me know how you would like to proceed further.

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Question

We double checked our constructs. On the left some his/thrombin tagged proteins stained with anti-HIS. On the right the same blot stained with your anti GST (stripped first)These proteins contain a thrombin site but no GST! Are you 100% sure you used GST isolated from Schistosoma japonicum as antigen?? It’s much easier/cheaper to express this protein recombinant in e.coli and then inject it in mice. But if you do it like that, often it contains a thrombin site because that’s introduced by the plasmid suppliers
Please let me know what you think..
Thanks
Kind regards,

Read More
Answer

Thank you for your email.

Laboratory has agreed to retest this antibody. I will get back to with more details soon.

Many thanks for having patience!

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Question

We are using your anti-GST antibody to detect our recombinant protein. Recently someone accidently used this antibody on a His-Trombin tagged protein (where a His-probe was supposed to be used). But he DID see a strong signal at the expected band size (of the rec. protein)!! We were wondering how this was possible (contamination, aspecific antibody, etc.). Therefore I was wondering what protein sequence was used for immunization?? I know it’s a free GST, but does this also contain a Thrombin sequence?

I hope you can retrieve these details for me

Already thanks

Kind regards,

Read More
Answer

Thank you for contacting us.

The immunogen sequence does not contain thrombin, it is a GST isolated from Schistosoma japonicum species. The antibody should not be binding to His conjugated proteins.

The band in His-thrombin might be a non specific binding which could be due to, high concentration of antibody used, damaged antibody or wrong storage of antibody. Could you let us know how the antibody was stored? Could you also repeat the experiment to check this further?

I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Inquiry: Hi, We have been very satisfied with the anti-GST HRP conjugated (rabbit polyclonal, ab3416) that we used for interaction studies. We would like to use an anti-GST texas red for immunostaining and found the following antibody # 106222 on your website. I have 2 questions regarding the anti-GST texas red (106222, rabbit polyclonal): - is it the same antibody preparation as ab3416, but Texas-Red conjugated? - the only application recorded for this antibody is WB. Would you recommend to use it for immunostaining? Thank you and best regards

Read More
Answer

Thank you for your enquiry. I am pleased to hear the ab3416 antibody has worked well in your experiments.

I can confirm that ab3416 and ab106222 are different antibodies.

I would like to reassure you that our antibodies are covered by our 6 month guarantee in the tested applications and species listed on the individual datasheets.

I am sorry to confirm that as far as we are aware, ab106222 has never been tested in IHC or ICC. All tested applications covered by our 6 month guarantee are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

If you would like to test ab106222 in ICC-IF, IHC-P (paraffin sections) or IHC-Fr (frozen sections), please contact me again prior to the purchase by replying to this message as you may be eligible for our testing discount program.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

Read More

Question

We have good experience using your ab3416 a-GST antibody. Now I would like to use this antibody without HRP conjugation. Could you please inform me about the abcam code for the original a-GST antibody.     

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Answer

Thank you for your question and for your patience while I worked to have this antibody added to our catalog. The unconjugated form of ab3416 is now available as ab115044- https://www.abcam.com/GST-antibody-ab115044.html Please let me know if you have any questions or if there is anything else that we can do for you.

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Question

I used ab3416 an HRP conjugated anti-GST polyclonal with success, and would like to translate my experiment to flow cytometry format with a FITC conjugated anti-GST. Is the ab3445 antibody from the same supplier/immunization as ab3416?

Read More
Answer

Sorry for the late response as the information you requested needed some double checking. I can confirm that the antibody preparation used for the FITC (ab3445) and HRP (ab3416) GST antibody is the same. I hope this confirmation will be helpful. If there is anything else that I can help you with, please do not hesitate to contact me.

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1-10 of 11 Abreviews or Q&A

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