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HRP Anti-GSTA1 antibody [8-326] (ab173836)

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Western blot - HRP Anti-GSTA1 antibody [8-326] (ab173836)

    Key features and details

    • HRP Mouse monoclonal [8-326] to GSTA1
    • Suitable for: WB
    • Reacts with: Human
    • Conjugation: HRP
    • Isotype: IgG1

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    Overview

    • Product name

      HRP Anti-GSTA1 antibody [8-326]
      See all GSTA1 primary antibodies
    • Description

      HRP Mouse monoclonal [8-326] to GSTA1
    • Host species

      Mouse
    • Conjugation

      HRP
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Recombinant full length protein corresponding to Human GSTA1 aa 1-250.
      Database link: P08263

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • Glutathione S Transferase alpha tagged fusion protein.
    • General notes

      Previously labelled as Glutathione S Transferase alpha 1. 

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Monoclonal
    • Clone number

      8-326
    • Isotype

      IgG1
    • Research areas

      • Signal Transduction
      • Metabolism
      • Energy Metabolism
      • Tags & Cell Markers
      • Fusion / Marker Proteins
      • GST
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Energy Metabolism
      • Metabolism
      • Pathways and Processes
      • Redox metabolism
      • Oxidative stress

    Associated products

    • Alternative Versions

      • Biotin Anti-GSTA1 antibody [8-326] (ab173835)
    • Recombinant Protein

      • Recombinant Human GSTA1 protein (ab95381)
    • Related Products

      • Recombinant human GSTA1 protein (ab167981)
      • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
      • TMB ELISA Substrate (High Sensitivity) (ab171523)
      • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
      • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
      • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
      • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
      • 450 nm Stop Solution for TMB Substrate (ab171529)
      • 650 nm Stop Solution for TMB Substrate (ab171531)
      • Immunoassay Blocking Buffer (ab171534)
      • Immunoassay Blocking (BSA Free) (ab171535)
      • Recombinant Human GSTA1 protein (ab95381)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab173836 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/500 - 1/2000. Predicted molecular weight: 26 kDa.
    Notes
    WB
    1/500 - 1/2000. Predicted molecular weight: 26 kDa.

    Target

    • Function

      Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
    • Tissue specificity

      Liver.
    • Sequence similarities

      Belongs to the GST superfamily. Alpha family.
      Contains 1 GST C-terminal domain.
      Contains 1 GST N-terminal domain.
    • Domain

      The C-terminal domain may form a component of the hydrophobic substrate-binding site, but in contrast appears not to be directly involved in GSH binding and is not absolutely essential for catalytic activity.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession P08263 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2938 Human
      • Omim: 138359 Human
      • SwissProt: P08263 Human
      • Unigene: 446309 Human
      • Alternative names

        • Glutathione S alkyltransferase A1 antibody
        • Glutathione S aryltransferase A1 antibody
        • Glutathione S transferase 2 antibody
        • Glutathione S transferase A1 antibody
        • Glutathione S transferase alpha 1 antibody
        • Glutathione S transferase Ha subunit 1 antibody
        • Glutathione S-transferase A1 antibody
        • GST class-alpha member 1 antibody
        • GST epsilon antibody
        • GST HA subunit 1 antibody
        • GST, class alpha, 1 antibody
        • GST-epsilon antibody
        • GST2 antibody
        • GSTA1 1 antibody
        • GSTA1 antibody
        • GSTA1-1 antibody
        • GSTA1_HUMAN antibody
        • GTH1 antibody
        • HA subunit 1 antibody
        • MGC131939 antibody
        • OTTHUMP00000016611 antibody
        • S (hydroxyalkyl)glutathione lyase A1 antibody
        see all

      Images

      • Western blot - HRP Anti-GSTA1 antibody [8-326] (ab173836)
        Western blot - HRP Anti-GSTA1 antibody [8-326] (ab173836)
        All lanes : HRP Anti-GSTA1 antibody [8-326] (ab173836) at 1/1000 dilution

        Lane 1 : purified GSTA1 -SRC SH2 domain at 5 µg
        Lane 2 : purified GSTA1 -SRC SH2 domain at 2.5 µg
        Lane 3 : purified GSTA1 -SRC SH2 domain at 1 µg
        Lane 4 : purified GSTA1 -SRC SH2 domain at 0.5 µg

        Developed using the ECL technique.

        Predicted band size: 26 kDa



        4-20% Tris-HCl polyacrylamide gel.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab173836? Please let us know so that we can cite the reference in this datasheet.

      ab173836 has not yet been referenced specifically in any publications.

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