HRP Anti-HA tag antibody [2-2.2.14] (ab173826)

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Western blot - HRP Anti-HA tag antibody [2-2.2.14] (ab173826)

    Key features and details

    • HRP Mouse monoclonal [2-2.2.14] to HA tag
    • Suitable for: WB
    • Reacts with: Species independent
    • Conjugation: HRP
    • Isotype: IgG1

    Overview

    • Product name

      HRP Anti-HA tag antibody [2-2.2.14]
      See all HA tag primary antibodies

    • Description

      HRP Mouse monoclonal [2-2.2.14] to HA tag

    • Host species

      Mouse

    • Conjugation

      HRP

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Species independent

    • Immunogen

      Synthetic peptide corresponding to HA tag aa 1-9 conjugated to Ovalbumin.
      Sequence:

      YPYDVPDYA

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • E. coli lysate containing a multi-epitope tagged protein.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab173826 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/2000. Predicted molecular weight: 1 kDa.

    Target

    • Relevance

      Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    • Alternative names

      • HA epitope tag antibody
      • HA1 antibody
      • HA2 antibody
      • hemagglutinin antibody
      • Hemagglutinin HA1 chain antibody
      • Hemagglutinin HA2 chain antibody
      see all

    Images

    • Western blot - HRP Anti-HA tag antibody [2-2.2.14] (ab173826)
      Western blot - HRP Anti-HA tag antibody [2-2.2.14] (ab173826)
      All lanes : HRP Anti-HA tag antibody [2-2.2.14] (ab173826) at 1/1000 dilution

      Lane 1 : E. coli lysate containing a multi-epitope tagged protein at 1 µg
      Lane 2 : E. coli lysate containing a multi-epitope tagged protein at 0.5 µg
      Lane 3 : E. coli lysate containing a multi-epitope tagged protein at 0.25 µg
      Lane 4 : E. coli lysate containing a multi-epitope tagged protein at 0.125 µg
      Lane 5 : E. coli lysate containing a multi-epitope tagged protein at 0.0500000007450581 µg

      Developed using the ECL technique.

      Predicted band size: 1 kDa



      Note: the predicted MW indicated is for the HA tag.

    References (1)

    Publishing research using ab173826? Please let us know so that we can cite the reference in this datasheet.

    ab173826 has been referenced in 1 publication.

    • Xia X  et al. A pseudo-receiver domain in Atg32 is required for mitophagy. Autophagy 14:1620-1628 (2018). PubMed: 29909755

    Customer reviews and Q&As

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    Western blot abreview for Anti-HA tag [2-2.2.14] antibody (HRP)

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (10)
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae cell lysate)
    Specification
    S. cerevisiae cell lysate
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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    Abcam user community

    Verified customer

    Submitted Mar 04 2014

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