HRP Anti-HA tag antibody (ab1190)

Submit a review Q&A (1)References (6)
ELISA - HRP Anti-HA tag antibody (ab1190)

    Key features and details

    • HRP Chicken polyclonal to HA tag
    • Suitable for: WB, ELISA
    • Reacts with: Species independent
    • Conjugation: HRP
    • Isotype: IgY

    Overview

    • Product name

      HRP Anti-HA tag antibody
      See all HA tag primary antibodies

    • Description

      HRP Chicken polyclonal to HA tag

    • Host species

      Chicken

    • Conjugation

      HRP

    • Specificity

      In a direct ELISA against 1 µg of HA peptide coupled to BSA as sample, conjugate diluted to 1/10,000 produced a 1.0 OD in a 15 minute reaction with TMB as substrate.

    • Tested applications

      Suitable for: WB, ELISAmore details

    • Species reactivity

      Reacts with: Species independent

    • Immunogen

      Synthetic peptide corresponding to HA tag conjugated to keyhole limpet haemocyanin. Corresponding to the influenza hemagglutinin-HA-epitope.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      pH: 7.00
      Preservative: 0.002% Thimerosal (merthiolate)
      Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to horseradish peroxidase.

    • Clonality

      Polyclonal

    • Isotype

      IgY

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab1190 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 0.05 - 0.5 µg/ml.
    ELISA
    Use a concentration of 1 - 0.2 µg/ml.
    Notes
    WB
    Use a concentration of 0.05 - 0.5 µg/ml.
    ELISA
    Use a concentration of 1 - 0.2 µg/ml.

    Target

    • Relevance

      Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    • Alternative names

      • HA epitope tag antibody
      • HA1 antibody
      • HA2 antibody
      • hemagglutinin antibody
      • Hemagglutinin HA1 chain antibody
      • Hemagglutinin HA2 chain antibody
      see all

    Images

    • ELISA - HRP Anti-HA tag antibody (ab1190)
      ELISA - HRP Anti-HA tag antibody (ab1190)

      ELISA - Anti-HA tag antibody (HRP) (ab1190).

    References (6)

    Publishing research using ab1190? Please let us know so that we can cite the reference in this datasheet.

    ab1190 has been referenced in 6 publications.

    • Li C  et al. Prophylactic In Vivo Hematopoietic Stem Cell Gene Therapy with an Immune Checkpoint Inhibitor Reverses Tumor Growth in Syngeneic Mouse Tumor Models. Cancer Res 80:549-560 (2020). PubMed: 31727629
    • Wang W  et al. TRIM37 deficiency induces autophagy through deregulating the MTORC1-TFEB axis. Autophagy 14:1574-1585 (2018). PubMed: 29940807
    • Scherer PC  et al. TRPV1 is a physiological regulator of µ-opioid receptors. Proc Natl Acad Sci U S A 114:13561-13566 (2017). PubMed: 29203659
    • van der Kant R  et al. Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity. J Biol Chem 290:30280-90 (2015). PubMed: 26463206
    • Klein CJ  et al. Ubiquitin ligase defect by DCAF8 mutation causes HMSN2 with giant axons. Neurology 82:873-8 (2014). PubMed: 24500646
    • Tchekanda E  et al. An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions. Nat Methods 11:641-4 (2014). PubMed: 24747815

    Customer reviews and Q&As

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    Question

    Hello, I am curious to konw if there is a clone name for this antibody? ab1190 - Chicken polyclonal to HA tag (HRP)

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    Answer

    Thank you for contacting us with your question. Since this antibody is a polyclonal, there is not a clone name associated with it. Only monoclonal antibodies will have a clone name. You can keep track of a polyclonal antibody based on their immunogen, host species, purity, or manufacturer and catalog number. I hope this information is helpful, but please let me know if you have any further questions.

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