HRP Anti-HA tag antibody (ab1190)
Key features and details
- HRP Chicken polyclonal to HA tag
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgY
Overview
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Product name
HRP Anti-HA tag antibody
See all HA tag primary antibodies -
Description
HRP Chicken polyclonal to HA tag -
Host species
Chicken -
Conjugation
HRP -
Specificity
In a direct ELISA against 1 µg of HA peptide coupled to BSA as sample, conjugate diluted to 1/10,000 produced a 1.0 OD in a 15 minute reaction with TMB as substrate.
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Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to HA tag conjugated to keyhole limpet haemocyanin. Corresponding to the influenza hemagglutinin-HA-epitope.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.00
Preservative: 0.002% Thimerosal (merthiolate)
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to horseradish peroxidase. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Related Products
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.05 - 0.5 µg/ml.
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| ELISA |
Use a concentration of 1 - 0.2 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 0.05 - 0.5 µg/ml. |
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ELISA
Use a concentration of 1 - 0.2 µg/ml. |
Target
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Relevance
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
Alternative names
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
Images
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ELISA - HRP Anti-HA tag antibody (ab1190)
ELISA - Anti-HA tag antibody (HRP) (ab1190).
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab1190 has been referenced in 5 publications.
- Wang W et al. TRIM37 deficiency induces autophagy through deregulating the MTORC1-TFEB axis. Autophagy 14:1574-1585 (2018). PubMed: 29940807
- Scherer PC et al. TRPV1 is a physiological regulator of µ-opioid receptors. Proc Natl Acad Sci U S A 114:13561-13566 (2017). PubMed: 29203659
- van der Kant R et al. Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity. J Biol Chem 290:30280-90 (2015). PubMed: 26463206
- Klein CJ et al. Ubiquitin ligase defect by DCAF8 mutation causes HMSN2 with giant axons. Neurology 82:873-8 (2014). PubMed: 24500646
- Tchekanda E et al. An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions. Nat Methods 11:641-4 (2014). PubMed: 24747815