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    hrp-ha-tag-antibody-ab183884.pdf

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Tags & Cell Markers Epitope Tags HA Tag
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HRP Anti-HA tag antibody (ab183884)

  • Datasheet
  • SDS
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Abpromise

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Find out more.

Western blot - HRP Anti-HA tag antibody (ab183884)

    Key features and details

    • HRP Rabbit polyclonal to HA tag
    • Suitable for: WB
    • Conjugation: HRP
    • Isotype: IgG

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    Conjugation
    Product image
    HRP Conjugation Kit - Lightning-Link® (ab102890)

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    Overview

    • Product name

      HRP Anti-HA tag antibody
      See all HA tag primary antibodies
    • Description

      HRP Rabbit polyclonal to HA tag
    • Host species

      Rabbit
    • Conjugation

      HRP
    • Tested applications

      Suitable for: WBmore details
    • Immunogen

      Synthetic peptide corresponding to HA tag aa 113-122 conjugated to Keyhole Limpet Haemocyanin (KLH).
      Sequence:

      YPYDVPDYA


      Database link: P03437
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • Transfected HeLa cells overexpressing HA-tagged protein.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.025% Proclin 300
      Constituents: 1% BSA, 0.88% Sodium chloride, 0.6% Tris
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Tags & Cell Markers
      • Epitope Tags
      • HA Tag

    Associated products

    • Related Products

      • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
      • TMB ELISA Substrate (High Sensitivity) (ab171523)
      • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
      • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
      • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
      • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
      • 450 nm Stop Solution for TMB Substrate (ab171529)
      • 650 nm Stop Solution for TMB Substrate (ab171531)
      • Immunoassay Blocking Buffer (ab171534)
      • Immunoassay Blocking (BSA Free) (ab171535)

    Applications

    Our Abpromise guarantee covers the use of ab183884 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/3000.

    Target

    • Relevance

      Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
    • Alternative names

      • HA epitope tag antibody
      • HA1 antibody
      • HA2 antibody
      • hemagglutinin antibody
      • Hemagglutinin HA1 chain antibody
      • Hemagglutinin HA2 chain antibody
      see all

    Images

    • Western blot - HRP Anti-HA tag antibody (ab183884)
      Western blot - HRP Anti-HA tag antibody (ab183884)
      All lanes : HRP Anti-HA tag antibody (ab183884) at 1/3000 dilution

      Lane 1 : Non-transfected HeLa cell lysate
      Lane 2 : Transfected HeLa cells over expressing HA-tagged protein lysate


      7.5% SDS PAGE

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab183884? Please let us know so that we can cite the reference in this datasheet.

    ab183884 has been referenced in 1 publication.

    • Li Q  et al. TRIM29 negatively controls antiviral immune response through targeting STING for degradation. Cell Discov 4:13 (2018). PubMed: 29581886

    Customer reviews and Q&As

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