HRP Anti-HA tag antibody (ab183884)

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Western blot - HRP Anti-HA tag antibody (ab183884)

    Key features and details

    • HRP Rabbit polyclonal to HA tag
    • Suitable for: WB
    • Conjugation: HRP
    • Isotype: IgG

    Overview

    • Product name

      HRP Anti-HA tag antibody
      See all HA tag primary antibodies

    • Description

      HRP Rabbit polyclonal to HA tag

    • Host species

      Rabbit

    • Conjugation

      HRP

    • Tested applications

      Suitable for: WBmore details

    • Immunogen

      Synthetic peptide corresponding to HA tag aa 100-200 conjugated to keyhole limpet haemocyanin.
      Database link: P03437

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • Transfected HeLa cells overexpressing HA-tagged protein.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle.

    • Storage buffer

      pH: 7.40
      Preservative: 0.025% Proclin 300
      Constituents: 1% BSA, 0.88% Sodium chloride, 0.6% Tris
    • Concentration information loading...

    • Purity

      Protein A purified

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab183884 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/3000.
    Notes
    WB
    1/3000.

    Target

    • Relevance

      Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    • Alternative names

      • HA epitope tag antibody
      • HA1 antibody
      • HA2 antibody
      • hemagglutinin antibody
      • Hemagglutinin HA1 chain antibody
      • Hemagglutinin HA2 chain antibody
      see all

    Images

    • Western blot - HRP Anti-HA tag antibody (ab183884)
      Western blot - HRP Anti-HA tag antibody (ab183884)
      All lanes : HRP Anti-HA tag antibody (ab183884) at 1/3000 dilution

      Lane 1 : Non-transfected HeLa cell lysate
      Lane 2 : Transfected HeLa cells over expressing HA-tagged protein lysate


      7.5% SDS PAGE

    References (1)

    Publishing research using ab183884? Please let us know so that we can cite the reference in this datasheet.

    ab183884 has been referenced in 1 publication.

    • Li Q  et al. TRIM29 negatively controls antiviral immune response through targeting STING for degradation. Cell Discov 4:13 (2018). PubMed: 29581886

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