Key features and details
- HRP Mouse monoclonal [3F3] to HDAC2 - C-terminal
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
- Isotype: IgG1
Product nameHRP Anti-HDAC2 antibody [3F3] - C-terminal
See all HDAC2 primary antibodies
DescriptionHRP Mouse monoclonal [3F3] to HDAC2 - C-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken
Storage instructionsShipped at 4°C. Store at +4°C. Store In the Dark.
Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab187941 in the following tested applications.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 55 kDa.|
FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.
Tissue specificityWidely expressed; lower levels in brain and lung.
Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
modificationsS-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.
- Information by UniProt
- D10Wsu179e antibody
- HD 2 antibody
- HD2 antibody
All lanes : HRP Anti-HDAC2 antibody [3F3] - C-terminal (ab187941) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : HDAC2 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 13 seconds
ab187941 was shown to specifically react with HDAC2 in wild-type HAP1 cells as signal was lost in HDAC2 knockout cells. Wild-type and HDAC2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab187941 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
ab187941 has not yet been referenced specifically in any publications.