Recombinant HRP Anti-HDGF antibody [EPR7898] (ab201820)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-HDGF antibody [EPR7898] (ab201820)](https://www.abcam.com/ps/products/201/ab201820/Images/ab201820-250613-IHCAP00220691.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-HDGF antibody [EPR7898] (ab201820)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR7898] to HDGF
- Suitable for: IHC-P, WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-HDGF antibody [EPR7898]
See all HDGF primary antibodies -
Description
HRP Rabbit monoclonal [EPR7898] to HDGF -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human HDGF aa 100-200. The exact sequence is proprietary.
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Positive control
- WB: A549 and SKBR-3 whole cell lysates. IHC-P: FFPE human placenta (normal) tissue sections.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7898 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab201820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 27 kDa). |
Target
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Function
Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the HDGF family.
Contains 1 PWWP domain. -
Domain
The PWWP domain harbors the heparin-binding sites and is responsible for DNA-binding, while the C-terminal region is essentially unstructured. -
Post-translational
modificationsSumoylated by SUMO1. Sumoylation prevents binding to chromatin. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3068 Human
- Entrez Gene: 15191 Mouse
- Entrez Gene: 114499 Rat
- Omim: 600339 Human
- SwissProt: P51858 Human
- SwissProt: P51859 Mouse
- SwissProt: Q8VHK7 Rat
- Unigene: 506748 Human
see all -
Alternative names
- FLJ96580 antibody
- HDGF antibody
- HDGF_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-HDGF antibody [EPR7898] (ab201820)
IHC image of HDGF staining in a section of formalin-fixed paraffin-embedded human placenta (normal)*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab201820, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - HRP Anti-HDGF antibody [EPR7898] (ab201820)](https://www.abcam.com/ps/products/201/ab201820/Images/ab201820-248806-WBab20182012m.jpg)
Western blot - HRP Anti-HDGF antibody [EPR7898] (ab201820)HRP Anti-HDGF antibody [EPR7898] (ab201820) at 1/1000 dilution + SKBR3 (Human breat adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab201820 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![Western blot - HRP Anti-HDGF antibody [EPR7898] (ab201820)](https://www.abcam.com/ps/products/201/ab201820/Images/ab201820-248802-WBab20182011m.jpg)
Western blot - HRP Anti-HDGF antibody [EPR7898] (ab201820)HRP Anti-HDGF antibody [EPR7898] (ab201820) at 1/1000 dilution + A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab201820 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-HDGF antibody [EPR7898] (ab201820)](https://www.abcam.com/ps/products/201/ab201820/Images/ab201820-5-benefits-of-recombinant-antibodies.png)
HRP Anti-HDGF antibody [EPR7898] (ab201820)
Protocols
References (0)
ab201820 has not yet been referenced specifically in any publications.
