HRP Anti-Hemagglutinin antibody (ab190060)
Key features and details
- HRP Chicken polyclonal to Hemagglutinin
- Suitable for: WB, ELISA
- Reacts with: Influenza A
- Conjugation: HRP
- Isotype: IgY
Overview
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Product name
HRP Anti-Hemagglutinin antibody
See all Hemagglutinin primary antibodies -
Description
HRP Chicken polyclonal to Hemagglutinin -
Host species
Chicken -
Conjugation
HRP -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Influenza A -
Immunogen
Synthetic peptide corresponding to Hemagglutinin conjugated to keyhole limpet haemocyanin.
Sequence:YPYDVPDYA
Database link: P03435 -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.00
Preservative: 0.005% Thimerosal (merthiolate)
Constituents: 0.6% HEPES, 0.29% Sodium chloride, 0.1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
After multiple immunizations in Freunds adjuvant, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support and the antibodies were conjugated to horseradish peroxidase (HRP). -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab190060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500. | |
| ELISA | 1/5000. The anti HA diluted 1:5,000 gives an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti peptide purification |
Target
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Relevance
Hemagglutinin (HA) is a class I viral fusion protein from Influenza virus. It is a major glycoprotein, comprising over 80% of the envelope proteins present in the virus particle. HA binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell, and is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. The extent of infection into host organism is determined by HA. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoproteases secreted by the bronchial epithelial cells. The HA protein is a homotrimer of disulfide-linked HA1-HA2. It also plays a major role in the determination of host range restriction and virulence. Genetic variation of hemagglutinin and/or neuraminidase genes results in the emergence of new influenza strains. -
Cellular localization
Cell membrane; apical cell membrane; single-pass type I membrane protein. -
Database links
- Entrez Gene: 3654620 Influenza A
- SwissProt: C3W5S1 Influenza A
- SwissProt: Q0HD60 Influenza A
- SwissProt: Q9Q0U6 Influenza A
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Alternative names
- HA antibody
- HA1 antibody
- HA2 antibody
- Hemagglutinin antibody
References (0)
ab190060 has not yet been referenced specifically in any publications.
