HRP Anti-Hemagglutinin antibody (ab190060)
Key features and details
- HRP Chicken polyclonal to Hemagglutinin
- Suitable for: WB, ELISA
- Reacts with: Influenza A
- Conjugation: HRP
- Isotype: IgY
Overview
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Product name
HRP Anti-Hemagglutinin antibody
See all Hemagglutinin primary antibodies -
Description
HRP Chicken polyclonal to Hemagglutinin -
Host species
Chicken -
Conjugation
HRP -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Influenza A -
Immunogen
Synthetic peptide corresponding to Influenza A Hemagglutinin aa 100-200 conjugated to keyhole limpet haemocyanin. Influenza A virus (strain A/Victoria/3/1975 H3N2)
Database link: P03435 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.00
Preservative: 0.005% Thimerosal (merthiolate)
Constituents: 0.6% HEPES, 0.29% Sodium chloride, 0.1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
After multiple immunizations in Freunds adjuvant, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support and the antibodies were conjugated to horseradish peroxidase (HRP). -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500.
|
|
| ELISA |
1/5000.
The anti HA diluted 1:5,000 gives an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti peptide purification |
| Notes |
|---|
|
WB
1/500. |
|
ELISA
1/5000. The anti HA diluted 1:5,000 gives an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti peptide purification |
Target
-
Relevance
Hemagglutinin (HA) is a class I viral fusion protein from Influenza virus. It is a major glycoprotein, comprising over 80% of the envelope proteins present in the virus particle. HA binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell, and is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. The extent of infection into host organism is determined by HA. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoproteases secreted by the bronchial epithelial cells. The HA protein is a homotrimer of disulfide-linked HA1-HA2. It also plays a major role in the determination of host range restriction and virulence. Genetic variation of hemagglutinin and/or neuraminidase genes results in the emergence of new influenza strains. -
Cellular localization
Cell membrane; apical cell membrane; single-pass type I membrane protein. -
Database links
- Entrez Gene: 3654620 Influenza A
- SwissProt: C3W5S1 Influenza A
- SwissProt: Q0HD60 Influenza A
- SwissProt: Q9Q0U6 Influenza A
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Alternative names
- HA antibody
- HA1 antibody
- HA2 antibody
- Hemagglutinin antibody
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab190060 has not yet been referenced specifically in any publications.