RecombinantRabMAb

Recombinant HRP Anti-Hemopexin antibody [EPR5610] (ab198556)

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Western blot - HRP Anti-Hemopexin antibody [EPR5610] (ab198556)
  • HRP Anti-Hemopexin antibody [EPR5610] (ab198556)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR5610] to Hemopexin
  • Suitable for: WB
  • Reacts with: Human
  • Conjugation: HRP

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free Unconjugated

Overview

  • Product name

    HRP Anti-Hemopexin antibody [EPR5610]
    See all Hemopexin primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR5610] to Hemopexin

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human Plasma Total Protein lysate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab198556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/5000. Detects a band of approximately 73 kDa (predicted molecular weight: 52 kDa).
Notes
WB
1/5000. Detects a band of approximately 73 kDa (predicted molecular weight: 52 kDa).

Target

  • Function

    Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.

  • Tissue specificity

    Expressed by the liver and secreted in plasma.

  • Sequence similarities

    Belongs to the hemopexin family.
    Contains 5 hemopexin-like domains.

  • Post-translational
    modifications

    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • Beta 1B glycoprotein antibody
      • Beta-1B-glycoprotein antibody
      • FLJ56652 antibody
      • HEMO antibody
      • HEMO_HUMAN antibody
      • Hemopexin antibody
      • Hpx antibody
      • HX antibody
      see all

    Images

    • Western blot - HRP Anti-Hemopexin antibody [EPR5610] (ab198556)
      Western blot - HRP Anti-Hemopexin antibody [EPR5610] (ab198556)
      HRP Anti-Hemopexin antibody [EPR5610] (ab198556) at 1/5000 dilution + Human Plasma Total Protein Lysate at 10 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 52 kDa
      Observed band size: 73 kDa why is the actual band size different from the predicted?


      Exposure time: 20 seconds


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab198556 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • HRP Anti-Hemopexin antibody [EPR5610] (ab198556)
      HRP Anti-Hemopexin antibody [EPR5610] (ab198556)

    References (0)

    Publishing research using ab198556? Please let us know so that we can cite the reference in this datasheet.

    ab198556 has not yet been referenced specifically in any publications.

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