Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR5610] to Hemopexin
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Product nameHRP Anti-Hemopexin antibody [EPR5610]
See all Hemopexin primary antibodies
DescriptionHRP Rabbit monoclonal [EPR5610] to Hemopexin
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Hemopexin aa 400-500. The exact sequence is proprietary.
- WB: Human Plasma Total Protein lysate.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Dissociation constant (KD)KD = 8.30 x 10 -12 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab198556 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 73 kDa (predicted molecular weight: 52 kDa).|
FunctionBinds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
Tissue specificityExpressed by the liver and secreted in plasma.
Sequence similaritiesBelongs to the hemopexin family.
Contains 5 hemopexin-like domains.
modificationsN- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
- Information by UniProt
- Beta 1B glycoprotein antibody
- Beta-1B-glycoprotein antibody
- FLJ56652 antibody
HRP Anti-Hemopexin antibody [EPR5610] (ab198556) at 1/5000 dilution + Human Plasma Total Protein Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted?
Exposure time: 20 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab198556 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab198556 has not yet been referenced specifically in any publications.