RecombinantRabMAb

Recombinant HRP Anti-Hemopexin antibody [EPR5610] (ab198556)

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Western blot - HRP Anti-Hemopexin antibody [EPR5610] (ab198556)
  • HRP Anti-Hemopexin antibody [EPR5610] (ab198556)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR5610] to Hemopexin
  • Suitable for: WB
  • Reacts with: Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-Hemopexin antibody [EPR5610]
    See all Hemopexin primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR5610] to Hemopexin

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Hemopexin aa 400-500. The exact sequence is proprietary.

  • Positive control

    • WB: Human Plasma Total Protein lysate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab198556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 73 kDa (predicted molecular weight: 52 kDa).

Target

  • Function

    Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.

  • Tissue specificity

    Expressed by the liver and secreted in plasma.

  • Sequence similarities

    Belongs to the hemopexin family.
    Contains 5 hemopexin-like domains.

  • Post-translational
    modifications

    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • Beta 1B glycoprotein antibody
      • Beta-1B-glycoprotein antibody
      • FLJ56652 antibody
      • HEMO antibody
      • HEMO_HUMAN antibody
      • Hemopexin antibody
      • Hpx antibody
      • HX antibody
      see all

    Images

    • Western blot - HRP Anti-Hemopexin antibody [EPR5610] (ab198556)
      Western blot - HRP Anti-Hemopexin antibody [EPR5610] (ab198556)
      HRP Anti-Hemopexin antibody [EPR5610] (ab198556) at 1/5000 dilution + Human Plasma Total Protein Lysate at 10 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 52 kDa
      Observed band size: 73 kDa
      why is the actual band size different from the predicted?


      Exposure time: 20 seconds


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab198556 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • HRP Anti-Hemopexin antibody [EPR5610] (ab198556)
      HRP Anti-Hemopexin antibody [EPR5610] (ab198556)

    References (0)

    Publishing research using ab198556? Please let us know so that we can cite the reference in this datasheet.

    ab198556 has not yet been referenced specifically in any publications.

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