HRP Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody (ab20878)
Key features and details
- HRP Rabbit polyclonal to Hepatitis B Virus Surface Antigen (Ad/Ay)
- Suitable for: ELISA
- Reacts with: Hepatitis B virus
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody
See all Hepatitis B Virus Surface Antigen (Ad/Ay) primary antibodies -
Description
HRP Rabbit polyclonal to Hepatitis B Virus Surface Antigen (Ad/Ay) -
Host species
Rabbit -
Conjugation
HRP -
Specificity
Monospecific, reacts only with Hepatitis B surface antigen including the pre-S1 epitope. Non-reactive with normal human serum. -
Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: Hepatitis B virus -
Immunogen
Hepatitis B surface antigen purified from human serum. Mixture of subtypes ad & ay.
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General notes
Estimated molar HRP:IgG substitution is 2-3.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.002% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Protein A chromatography purified IgG fraction covalently coupled to a highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Applications
Our Abpromise guarantee covers the use of ab20878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Suitability for use in Western blot, IHC and IFA has not been determined but use in such assays should not be excluded.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr. -
Cellular localization
Virion membrane -
Database links
- Entrez Gene: 2828293 Hepatitis B virus
- Entrez Gene: 944569 Hepatitis B virus
- SwissProt: P03138 Hepatitis B virus
- SwissProt: P03140 Hepatitis B virus
- SwissProt: P03141 Hepatitis B virus
- SwissProt: Q81157 Hepatitis B virus
- SwissProt: Q81158 Hepatitis B virus
- SwissProt: Q81159 Hepatitis B virus
see all -
Alternative names
- HBsAg antibody
- HBV major surface antigen antibody
- HBV surface antigen antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab20878 has not yet been referenced specifically in any publications.