HRP Anti-Hepatitis B Virus Surface Antigen antibody (ab19990)

Submit a review Q&A (2)

Key features and details

  • HRP Goat polyclonal to Hepatitis B Virus Surface Antigen
  • Suitable for: ELISA
  • Reacts with: Hepatitis B virus
  • Conjugation: HRP
  • Isotype: IgG

Overview

  • Product name

    HRP Anti-Hepatitis B Virus Surface Antigen antibody
    See all Hepatitis B Virus Surface Antigen primary antibodies

  • Description

    HRP Goat polyclonal to Hepatitis B Virus Surface Antigen

  • Host species

    Goat

  • Conjugation

    HRP

  • Tested applications

    Suitable for: ELISAmore details

  • Species reactivity

    Reacts with: Hepatitis B virus

  • Immunogen

    Hepatitis B surface antigen. Mixture of subtypes ad & ay.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab19990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.

Target

  • Relevance

    Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.

  • Cellular localization

    Virion membrane

  • Database links

    • Alternative names

      • Large envelope protein antibody
      • Large surface protein antibody
      • LHB antibody
      • Major surface antigen antibody

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab19990? Please let us know so that we can cite the reference in this datasheet.

    ab19990 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    I tried standard protocol (bicarb pH9.6 coating, blocking 1% BSA, then sandwich with your sAg standard, and detection with 2nd goat anti mouse HRP).

    I ordered this Ab pair you suggested. Can you pls give me the protocol details that have worked for Abcam? I'll be using the same sAg standard that I ordered before.

    Read More
    Answer

    Here are a few specific sELISA protocol details I was able to find:

    ab2039, the capture antibody, was coated on the plate at 5 μg/ml in 0.1 M carbonate buffer, and the HRP-conjugated detection antibody, ab2042, was used at 0.5-5 ug/mL.

    Otherwise, a standard sELISA protocol such as https://www.abcam.com/index.html?pageconfig=resource&rid=11422 (or your current in-house protocol) can be used with appropriate negative controls. One thing I could not find was what source for Hepatitis B Virus Surface Antigen was used in this sELISA.

    I hope this is helpful. Please contact me again if you have any further questions.

    Use our products? Submit an https://www.abcam.com/abreviews. Earn rewards!

    Read More

    Question

    Inquiry: I purchased two anti HBsAg antibodies (ab9193 and ab8636) and ab113610 protein as standard but don't see any signal in my ELISA attempt. Which antibody pairs have you tested for ELISA? Can you recommend a detection Ab that I can use with ab9193, e.g. HRP conjugated ab19990 or ab2042?

    Read More
    Answer



    If you don't mind providing a more complete protocol for the ELISA assay you ran, I can help you to troubleshoot further.

    We have already tested ab2039 to work successfully with ab2042 in sandwich ELISA.

    We have not yet determined which detection antibodies may work with ab9193 in and ELISA.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com