HRP Anti-Hepatitis B Virus Surface Antigen antibody (ab19990)

Submit a review Q&A (2)

Key features and details

  • HRP Goat polyclonal to Hepatitis B Virus Surface Antigen
  • Suitable for: ELISA
  • Reacts with: Hepatitis B virus
  • Conjugation: HRP
  • Isotype: IgG

Overview

  • Product name

    HRP Anti-Hepatitis B Virus Surface Antigen antibody
    See all Hepatitis B Virus Surface Antigen primary antibodies

  • Description

    HRP Goat polyclonal to Hepatitis B Virus Surface Antigen

  • Host species

    Goat

  • Conjugation

    HRP

  • Tested applications

    Suitable for: ELISAmore details

  • Species reactivity

    Reacts with: Hepatitis B virus

  • Immunogen

    Full length native protein (purified) corresponding to Hepatitis B Virus Surface Antigen. Mixture of subtypes ad & ay.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab19990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
1/1000.
Notes
ELISA
1/1000.

Target

  • Relevance

    Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.

  • Cellular localization

    Virion membrane

  • Database links

    • Alternative names

      • Large envelope protein antibody
      • Large surface protein antibody
      • LHB antibody
      • Major surface antigen antibody

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab19990? Please let us know so that we can cite the reference in this datasheet.

    ab19990 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    I tried standard protocol (bicarb pH9.6 coating, blocking 1% BSA, then sandwich with your sAg standard, and detection with 2nd goat anti mouse HRP).

    I ordered this Ab pair you suggested. Can you pls give me the protocol details that have worked for Abcam? I'll be using the same sAg standard that I ordered before.

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    Answer

    Here are a few specific sELISA protocol details I was able to find:

    ab2039, the capture antibody, was coated on the plate at 5 μg/ml in 0.1 M carbonate buffer, and the HRP-conjugated detection antibody, ab2042, was used at 0.5-5 ug/mL.

    Otherwise, a standard sELISA protocol such as https://www.abcam.com/index.html?pageconfig=resource&rid=11422 (or your current in-house protocol) can be used with appropriate negative controls. One thing I could not find was what source for Hepatitis B Virus Surface Antigen was used in this sELISA.

    I hope this is helpful. Please contact me again if you have any further questions.

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    Question

    Inquiry: I purchased two anti HBsAg antibodies (ab9193 and ab8636) and ab113610 protein as standard but don't see any signal in my ELISA attempt. Which antibody pairs have you tested for ELISA? Can you recommend a detection Ab that I can use with ab9193, e.g. HRP conjugated ab19990 or ab2042?

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    Answer



    If you don't mind providing a more complete protocol for the ELISA assay you ran, I can help you to troubleshoot further.

    We have already tested ab2039 to work successfully with ab2042 in sandwich ELISA.

    We have not yet determined which detection antibodies may work with ab9193 in and ELISA.

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