HRP Anti-HIV1 gp120 antibody (ab20008)

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Key features and details

  • HRP Goat polyclonal to HIV1 gp120
  • Suitable for: WB, ELISA
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgG

Overview

  • Product name

    HRP Anti-HIV1 gp120 antibody
    See all HIV1 gp120 primary antibodies

  • Description

    HRP Goat polyclonal to HIV1 gp120

  • Host species

    Goat

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WB, ELISAmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Purified native gp120 from HIV strain IIIB.

  • General notes

    IgG fraction covalently coupled to a highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Estimated molar HRP: IgG substitution is 2-3.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab20008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: 1/200 - 1/1000. WB: 1/20 - 1/200. Predicted molecular weight: 120 kDa. Dilution optimised using Chromogenic detection. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Relevance

      HIV1 is equipped with the envelope gp160 glycoprotein for interaction with Langerhans cells (LCs) and dendritic cells (DCs), the members of the innate immune system, which confront the virus at the portal of virus entry in the human body. These cells are equipped with receptors by which they bind and endocytose the virus. The gp120 glycoprotein is used for binding to CD4 receptor and CCR5 co-receptor of T helper 2 (Th2) cells, and is able to induce FcepsilonRI(+) hematopoietic cells to produce IL4, which inactivates the host adaptive immune response.

    • Cellular localization

      Envelope surface glycoprotein.

    • Alternative names

      • Glycoprotein 120 antibody
      • gp120 antibody
      • SU antibody
      • Surface protein antibody

    References (0)

    Publishing research using ab20008? Please let us know so that we can cite the reference in this datasheet.

    ab20008 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Question

    Product code: 20008

    Inquiry: Hi, We are wondering whether this Ab (Anti-HIV1 gp120 antibody (HRP) (ab20008)) would react with a recombinant truncated gp120 that binds CD4 and CCR5, but lacks V1, V2, V3 and the gp41 binding domain. Thank you,

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    Answer

    Thank you for your inquiry.

    I can confirm that the immunogen for ab20008is purified native gp120 from HIV strain IIIB.

    Since it is a polyclonal antibody we do not know where exactly it bind in the protein.

    We have one mouse monoclonal antibody where the immunogen is is now and a short peptide sequence: ab24745, WQEVGKAMYAPPISGQIRCSSN, corresponding to amino acids 427-448 of Human HIV type I (BH8 isolate) gp120

    https://www.abcam.com/index.html?datasheet=24745 (or use the following: https://www.abcam.com/index.html?datasheet=24745).

    I am sorry that I could not answer your question and hope this information is nevertheless helpful.

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    Question

    Can you tell me if these products are from the same origin (ab21179, ab20105, ab20262, ab20008, ab53840, ab53937).I am looking for the non-conjugated form of ab53937 and ab20105.

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    Answer

    Thank you for your enquiry. These 4 products (ab21179, ab20105, ab20262, ab20008) were produced from the same goat antiserum. Catalog ab21179 is the purified antibody. The purified antibody is then labeled to produce the other catalog numbers: Biotin (ab20105), FITC (ab20262), HRP (ab20008). Meanwhile, ab53840 and ab53937 originate from the same goat antisera. Unfortunately, we do not have the unconjugated version of these antibodies in our catalog but I may check with the originator if it is available. Please let me know if you would like to proceed with an order.

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    Question

    Abs to be used in ELISA, WB against the HIV strain YU2 Gp41: ab20890 ab18633 ab19975 ab24714 Gp120: ab24745 ab20008 ab20105

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    Answer

    Thank you for your enquiry. I have been in touch with the source of these HIV gp120 and gp41 antibodies and I have determine that unfortunately none of them have been tested against the HIV strain YU2. I can tell you that ab18633 is predicted, based on broad reactivity to react with HIV strain YU2. I am sorry that I cannot be of more assistance on this occasion.

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