HRP Anti-HIV1 gp120 antibody (ab53840)

Reviews (1)Q&A (1)References (1)

Key features and details

  • HRP Goat polyclonal to HIV1 gp120
  • Suitable for: WB, ELISA, IHC-Fr
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgG

Overview

  • Product name

    HRP Anti-HIV1 gp120 antibody
    See all HIV1 gp120 primary antibodies

  • Description

    HRP Goat polyclonal to HIV1 gp120

  • Host species

    Goat

  • Conjugation

    HRP

  • Specificity

    Gp120 is the predominant band in WB.

  • Tested applications

    Suitable for: WB, ELISA, IHC-Frmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Full length native protein, from strain IIIB.

  • General notes

    Estimated molar HRP:IgG substitution is 2-3. Free enzyme is absent.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab53840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/20 - 1/200.
ELISA
1/200 - 1/1000.
IHC-Fr
1/20 - 1/200.
Notes
WB
1/20 - 1/200.
ELISA
1/200 - 1/1000.
IHC-Fr
1/20 - 1/200.

Target

  • Relevance

    HIV1 is equipped with the envelope gp160 glycoprotein for interaction with Langerhans cells (LCs) and dendritic cells (DCs), the members of the innate immune system, which confront the virus at the portal of virus entry in the human body. These cells are equipped with receptors by which they bind and endocytose the virus. The gp120 glycoprotein is used for binding to CD4 receptor and CCR5 co-receptor of T helper 2 (Th2) cells, and is able to induce FcepsilonRI(+) hematopoietic cells to produce IL4, which inactivates the host adaptive immune response.

  • Cellular localization

    Envelope surface glycoprotein.

  • Alternative names

    • Glycoprotein 120 antibody
    • gp120 antibody
    • SU antibody
    • Surface protein antibody

References (1)

Publishing research using ab53840? Please let us know so that we can cite the reference in this datasheet.

ab53840 has been referenced in 1 publication.

  • Grimm SK  et al. Directed evolution of a yeast-displayed HIV-1 SOSIP gp140 spike protein toward improved expression and affinity for conformational antibodies. PLoS One 10:e0117227 (2015). WB . PubMed: 25688555

Customer reviews and Q&As

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Western blot abreview for Anti-HIV1 gp120 antibody (HRP)

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (3-8% Tris-acetate SDS-PAGE)
Sample
Human Cell lysate - other (HOS)
Specification
HOS
Treatment
transfected with HIV-1 Env expression plasmid
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Oct 17 2014

Question

Can you tell me if these products are from the same origin (ab21179, ab20105, ab20262, ab20008, ab53840, ab53937).I am looking for the non-conjugated form of ab53937 and ab20105.

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Answer

Thank you for your enquiry. These 4 products (ab21179, ab20105, ab20262, ab20008) were produced from the same goat antiserum. Catalog ab21179 is the purified antibody. The purified antibody is then labeled to produce the other catalog numbers: Biotin (ab20105), FITC (ab20262), HRP (ab20008). Meanwhile, ab53840 and ab53937 originate from the same goat antisera. Unfortunately, we do not have the unconjugated version of these antibodies in our catalog but I may check with the originator if it is available. Please let me know if you would like to proceed with an order.

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