HRP Anti-HIV1 gp41 antibody (ab68622)

Submit a review Q&A (1)References (1)

Key features and details

  • HRP Goat polyclonal to HIV1 gp41
  • Suitable for: WB, ELISA
  • Conjugation: HRP
  • Isotype: IgG

Overview

  • Product name

    HRP Anti-HIV1 gp41 antibody
    See all HIV1 gp41 primary antibodies

  • Description

    HRP Goat polyclonal to HIV1 gp41

  • Host species

    Goat

  • Conjugation

    HRP

  • Specificity

    This antibody reacts with HIV1 gp41 in viral and infected tissue samples.

  • Tested applications

    Suitable for: WB, ELISAmore details

  • Species reactivity

    Reacts with: Other species

  • Immunogen

    Recombinant fragment, corresponding to the ectodomain of gp41-glycosylated (a.a. residues 546-682) from strain HxB2.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab68622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.
ELISA
1/200 - 1/1000.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.
ELISA
1/200 - 1/1000.

Target

  • Relevance

    gp41/120 is the major HIV protein associated with the HIV envelope. It functions as the viral antireceptor or attachment protein. gp41 (or TM) traverses the envelope, whereas gp120 is present on the outer surface and is noncovalently attached to gp41. The precursor of gp120/41 (gp160) is synthesized in the endoplasmic reticulum and is transported via the golgi body to the cell surface. Upon activation of the envelope glycoprotein (gp120/41) by cellular receptors, gp41 undergoes conformational changes that mediate fusion of the viral and cellular membranes.

  • Alternative names

    • Env antibody
    • Glycoprotein 41 antibody
    • gp41 antibody
    • Human Immunodeficiency Virus 1 antibody
    • TM antibody
    • Transmembrane protein antibody
    see all

References (1)

Publishing research using ab68622? Please let us know so that we can cite the reference in this datasheet.

ab68622 has been referenced in 1 publication.

  • Zhou X  et al. Alpha-1-antitrypsin interacts with gp41 to block HIV-1 entry into CD4+ T lymphocytes. BMC Microbiol 16:172 (2016). WB ; Human . PubMed: 27473095

Customer reviews and Q&As

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Question

Hello,

We would like to immobilize Anti-HIV1 gp120 onto a surface to show efficacy in capturing HIV protein.

Our current thought is to:
1. Immobilize Anti-HIV1 gp120 antibody (Product # ab21179)
2.Capture HIV1 gp120 + gp41 protein (Product #ab68127)
3. Use the secondary protein, Anti-HIV1 gp41 (Product # ab68622) conjugated to HRP to measure and quantify the amount of captured protein.

Can you confirm that the three products (ab21179, ab68127, and ab68622) will interact as predicted?

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Answer

Thank you for contacting us. Theoretically your outline makes sense. However, we have not tested the products this way and cannot guarantee that the binding sites of the antibodies will not interfere with each other. I hope this is helpful. Please le me know if I can be of further assistance.

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