HRP Anti-HIV1 p24 antibody (ab20365)

Reviews (1)Q&A (1)References (2)

Key features and details

  • HRP Goat polyclonal to HIV1 p24
  • Suitable for: ELISA, ICC, WB
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgG

Overview

  • Product name

    HRP Anti-HIV1 p24 antibody
    See all HIV1 p24 primary antibodies

  • Description

    HRP Goat polyclonal to HIV1 p24

  • Host species

    Goat

  • Conjugation

    HRP

  • Specificity

    HIV1 p24 antibody (ab20365) targets p24 (predominate p24 band pattern in Western blot). Does not cross-react with human T or B cells. Antiserum is not reactive with human or bovine sera.

  • Tested applications

    Suitable for: ELISA, ICC, WBmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Full length native protein (purified native p24 from strain IIIb).

  • General notes

    Purified IgG fraction covalently coupled to a highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab20365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA (1)
1/200 - 1/1000.
ICC
1/20 - 1/200.
WB
1/200 - 1/1000. Predicted molecular weight: 56 kDa. Dilution optimised using Chromogenic detection. When using chemiluminescent substrates, the assay will require much less antibody. Depending on the sensitivity of the substrate, the antibody dilution may be 1/10,000 - 1/100,000.
Notes
ELISA
1/200 - 1/1000.
ICC
1/20 - 1/200.
WB
1/200 - 1/1000. Predicted molecular weight: 56 kDa. Dilution optimised using Chromogenic detection. When using chemiluminescent substrates, the assay will require much less antibody. Depending on the sensitivity of the substrate, the antibody dilution may be 1/10,000 - 1/100,000.

Target

  • Relevance

    HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.

  • Cellular localization

    Membrane

  • Alternative names

    • CA antibody
    • Capsid protein p24 antibody
    • HIV-1 Gag p24 antibody
    • HIV1gp1 antibody
    • Human immunodeficiency virus 1 antibody
    • Human immunodeficiency virus type 1 p24 antibody
    see all

References (2)

Publishing research using ab20365? Please let us know so that we can cite the reference in this datasheet.

ab20365 has been referenced in 2 publications.

  • Peyret H  et al. Improving plant transient expression through the rational design of synthetic 5' and 3' untranslated regions. Plant Methods 15:108 (2019). PubMed: 31548848
  • Guth CA & Sodroski J Contribution of PDZD8 to stabilization of the human immunodeficiency virus type 1 capsid. J Virol 88:4612-23 (2014). ELISA . PubMed: 24554657

Customer reviews and Q&As

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ELISA abreview for Anti-HIV1 p24 antibody (HRP)

 Excellent
Abreviews
abreview image
Application
ELISA
Sample
HIV Purified protein (purified from bacteria overexpression)
Specification
purified from bacteria overexpression
Type
Direct
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
Read More

Dr. Shijian Zhang

Verified customer

Submitted Jun 03 2014

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