HRP Anti-HIV1 p24 antibody (ab20365)

Reviews (1)Q&A (1)References (2)

Key features and details

  • HRP Goat polyclonal to HIV1 p24
  • Suitable for: ELISA, ICC, WB
  • Reacts with: Species independent
  • Conjugation: HRP
  • Isotype: IgG

Overview

  • Product name

    HRP Anti-HIV1 p24 antibody
    See all HIV1 p24 primary antibodies

  • Description

    HRP Goat polyclonal to HIV1 p24

  • Host species

    Goat

  • Conjugation

    HRP

  • Specificity

    HIV1 p24 antibody (ab20365) targets p24 (predominate p24 band pattern in Western blot). Does not cross-react with human T or B cells. Antiserum is not reactive with human or bovine sera.

  • Tested applications

    Suitable for: ELISA, ICC, WBmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Full length native protein (purified native p24 from strain IIIb).

  • General notes

    Purified IgG fraction covalently coupled to a highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab20365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/200 - 1/1000.
ICC 1/20 - 1/200.
WB 1/200 - 1/1000. Predicted molecular weight: 56 kDa. Dilution optimised using Chromogenic detection. When using chemiluminescent substrates, the assay will require much less antibody. Depending on the sensitivity of the substrate, the antibody dilution may be 1/10,000 - 1/100,000.

Target

  • Relevance

    HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.

  • Cellular localization

    Membrane

  • Alternative names

    • CA antibody
    • Capsid protein p24 antibody
    • HIV-1 Gag p24 antibody
    • HIV1gp1 antibody
    • Human immunodeficiency virus 1 antibody
    • Human immunodeficiency virus type 1 p24 antibody
    see all

References (2)

Publishing research using ab20365? Please let us know so that we can cite the reference in this datasheet.

ab20365 has been referenced in 2 publications.

  • Peyret H  et al. Improving plant transient expression through the rational design of synthetic 5' and 3' untranslated regions. Plant Methods 15:108 (2019). PubMed: 31548848
  • Guth CA & Sodroski J Contribution of PDZD8 to stabilization of the human immunodeficiency virus type 1 capsid. J Virol 88:4612-23 (2014). ELISA . PubMed: 24554657

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

ELISA abreview for Anti-HIV1 p24 antibody (HRP)

 Excellent
Abreviews
abreview image
Application
ELISA
Sample
HIV Purified protein (purified from bacteria overexpression)
Specification
purified from bacteria overexpression
Type
Direct
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
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Dr. Shijian Zhang

Verified customer

Submitted Jun 03 2014

Question

I'm doing an ELISPOT-type assay with these products and am looking for a protocol.

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Answer

Thank you for your inquiry. I know that you said you're using nitrocellulose, and this protocol uses PVDF, but I hope you can adjust it accordingly. https://www.abcam.com/index.html?pageconfig=resource&rid=11456 I hope this information helps. Please contact us with any other questions.

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