HRP Anti-hnRNP K antibody [F45 P9 C7] (ab204456)
Key features and details
- HRP Mouse monoclonal [F45 P9 C7] to hnRNP K
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
HRP Anti-hnRNP K antibody [F45 P9 C7]
See all hnRNP K primary antibodies -
Description
HRP Mouse monoclonal [F45 P9 C7] to hnRNP K -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Chicken -
Immunogen
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Positive control
- WB: HEK293, NIH3T3 and PC12 whole cell lysates. IHC-P: FFPE Human Kidney Normal
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
F45 P9 C7 -
Myeloma
Ag8.653 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 64 kDa (predicted molecular weight: 51 kDa).
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IHC-P |
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/5000. Detects a band of approximately 64 kDa (predicted molecular weight: 51 kDa). |
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. -
Sequence similarities
Contains 3 KH domains. -
Post-translational
modificationsArg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Cytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear. - Information by UniProt
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Database links
- Entrez Gene: 3190 Human
- Entrez Gene: 15387 Mouse
- Entrez Gene: 100008849 Rabbit
- Entrez Gene: 117282 Rat
- Omim: 600712 Human
- SwissProt: Q5ZIQ3 Chicken
- SwissProt: P61978 Human
- SwissProt: P61979 Mouse
see all -
Alternative names
- CSBP antibody
- dC stretch binding protein antibody
- FLJ41122 antibody
see all
Images
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IHC image of hnRNP K staining in a section of formalin-fixed paraffin-embedded Normal Human Kidney*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab204456, 1/50 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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All lanes : HRP Anti-hnRNP K antibody [F45 P9 C7] (ab204456) at 1/5000 dilution
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab204456 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab204456 has been referenced in 3 publications.
- Pham T et al. Multiplexed In Situ Protein Profiling with High-Performance Cleavable Fluorescent Tyramide. Molecules 26:N/A (2021). PubMed: 33921211
- Xiao L et al. Highly Sensitive and Multiplexed In Situ RNA Profiling with Cleavable Fluorescent Tyramide. Cells 10:N/A (2021). PubMed: 34063986
- Liao R et al. Highly Sensitive and Multiplexed Protein Imaging With Cleavable Fluorescent Tyramide Reveals Human Neuronal Heterogeneity. Front Cell Dev Biol 8:614624 (2020). PubMed: 33585449