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  1. Link

    hrp-human-serum-albumin-antibody-ab19183.pdf

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Cardiovascular Blood Serum Proteins
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HRP Anti-Human Serum Albumin antibody (ab19183)

  • Datasheet
  • SDS
Reviews (1)Q&A (6)References (1)

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Abpromise

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Key features and details

  • HRP Goat polyclonal to Human Serum Albumin
  • Reacts with: Human, Macaque monkey
  • Conjugation: HRP
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human Serum Albumin protein (His tag) (ab217817)

View more associated products

Overview

  • Product name

    HRP Anti-Human Serum Albumin antibody
    See all Human Serum Albumin primary antibodies
  • Description

    HRP Goat polyclonal to Human Serum Albumin
  • Host species

    Goat
  • Conjugation

    HRP
  • Specificity

    ab19183 was tested by ELISA against the following: Mouse reference serum, bovine serum albumin - undetectable cross reactivity, purified mouse albumin and rhesus monkey serum - cross reactivity detected.
  • Species reactivity

    Reacts with: Human, Macaque monkey
  • Immunogen

    Unfortunately, this information is considered to be commercially sensitive

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 6.8
    Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was solid phase adsorbed to ensure specificity.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Serum Proteins
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Recombinant Protein

    • Recombinant Human Serum Albumin protein (His tag) (ab217817)
  • Related Products

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
    • 450 nm Stop Solution for TMB Substrate (ab171529)
    • 650 nm Stop Solution for TMB Substrate (ab171531)
    • Immunoassay Blocking Buffer (ab171534)
    • Immunoassay Blocking (BSA Free) (ab171535)

Target

  • Function

    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • Tissue specificity

    Plasma.
  • Involvement in disease

    Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • Sequence similarities

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational
    modifications

    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P02768 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 213 Human
    • Omim: 103600 Human
    • SwissProt: P02768 Human
    • Unigene: 418167 Human
    • Alternative names

      • alb antibody
      • ALBU_HUMAN antibody
      • Albumin (32 AA) antibody
      • Albumin (AA 34) antibody
      • Albumin antibody
      • Analbuminemia antibody
      • Bisalbuminemia antibody
      • Cell growth inhibiting protein 42 antibody
      • DKFZp779N1935 antibody
      • Dysalbuminemic hyperthyroxinemia antibody
      • Growth inhibiting protein 20 antibody
      • HSA antibody
      • Hyperthyroxinemia dysalbuminemic antibody
      • PRO0883 antibody
      • PRO0903 antibody
      • PRO1341 antibody
      • Serum albumin antibody
      see all

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab19183? Please let us know so that we can cite the reference in this datasheet.

    ab19183 has been referenced in 1 publication.

    • Taniguchi M  et al. Multimolecular salivary mucin complex is altered in saliva of cigarette smokers: detection of disulfide bridges by Raman spectroscopy. Biomed Res Int 2013:168765 (2013). WB ; Human . PubMed: 23509686

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-7 of 7 Abreviews or Q&A

    ELISA abreview for Anti-Human Serum Albumin antibody (HRP)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Mouse Serum (ICR Mouse serum)
    Specification
    ICR Mouse serum
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Type
    Sandwich (Detection)
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 24 2019

    Question


    I am not sure how to calibrate the ELSIA. When I prepare a series of standard protein solutions, do I need to treat the containers first , e.g. coating the BSA on the micro-centrifuge tubes? Because the concentration of standard protein solution is very low, any adsorption of proteins on the container or the tips will change the concentration. Maybe I am wrong, such adsorption is very small or zero and can be neglected .

    Read More

    Abcam community

    Verified customer

    Asked on Apr 11 2012

    Answer

    If you would like to coat the microtiter plate wells with your protein target, you should do that first. Adding BSA after allowing the protein target to bind the wells serves to block the wells from binding any more protein (ie. the antibodies).

    Here is a link to an ELISA protocol from our protocols section which may be useful:

    https://www.abcam.com/index.html?pageconfig=resource&rid=11388

    Read More

    Abcam Scientific Support

    Answered on Apr 11 2012

    Question

    I have ordered these HRP conjugated antibodies, ab7539, ab19183, and ab25467 today. Once I received these antibodies, I plan to aliquot and store them in the fridge. Since its total volume is small (100 ul), I want to dilute these by 1:10 first and then aliquot. I am wondering what the dilution solution can be used, can I use the PBS buffer only? If not, What do I need to add? Thank you.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 04 2012

    Answer

    We would not recommend diluting the antibodies before storing. Please follow the instructions as listed on each antibody's datasheet:

    For ab19183, store the whole vial at 4C.

    For ab25467 and ab31790, store at +4°C short term (1-2 weeks). Aliquot (intono smaller than 10ul)and store at -20°C long term. Avoid repeated freeze / thaw cycles.

    More information can be found in our Antibody storage guide. Please note that improperly stored antibodies can void the Abpromise since we don't test our antibodies in all types of storage conditions, and therefore do not know how these will impact antibody quality.

    https://www.abcam.com/index.html?pageconfig=resource&rid=10795

    Read More

    Abcam Scientific Support

    Answered on Apr 04 2012

    Question

    Is there any protocol of ELISA you can share?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 02 2012

    Answer

    You can find our recommended ELISA protocols here:

    https://www.abcam.com/index.html?pageconfig=resource&rid=11388

    Read More

    Abcam Scientific Support

    Answered on Apr 02 2012

    Question

    I would like to use antibody conjugated with HRP to measure proteins by ELISA .

    We are interested in quantification of each protein amount adsorbed on biomaterial surface from blood or human plasma, specifically focusing on fibrinogen and fibronectin, as well as albumin. I am concerned about the interference such as cross reactivity. For example, when I measure the fibrinogen, is there any interference from fibronectin?

    It will be great if I can use three antibody at same time. If not possible, I will measure the proteins separately.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 30 2012

    Answer

    I would recommend the following antibodies for your experiments:


    ab7539 - Anti-Fibrinogen (HRP)

    ab19183 - Anti - Human Serum Albumin (HRP)

    ab25467 - Anti -Fibronectin (HRP)


    Each are conjugated to HRP and do not require a secondary antibody, however, they are all conjugated to the same enzyme and thus each one must be detected in a separate well as you would not be able to distinguish one protein from another in the same well.

    Read More

    Abcam Scientific Support

    Answered on Mar 30 2012

    Question

    Can you provide any information about how many HRP molecules are conjugated to IgG molecules and the QC method to IgG-HRP?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 06 2012

    Answer

    The first step of the conjugation method involves the oxidation of the sugar moieties of HRP into reactive aldehyde groups. In the second step, the aldehyde groups of HRP are allowed to form irreversible covalent bonds with some of the amino groups of the antibody. Since the reaction mixture is a 1:1 ratio of the antibody to HRP, the final molar ratio calculates to 3-4 HRP molecules per antibody molecule. The conjugate is assayed functionally through direct or sandwich Elisa assay (depending on the antibody), and compared with the previous one or two lots (conjugation batches).

    Read More

    Abcam Scientific Support

    Answered on Jan 06 2012

    Question

    Bonjour, Je serais eventuellement interessee par votre anticorps primaire ab10241 anti-human serum albumin (clone 15C7, mouse monoclonal). Mais j'aurais besoin de savoir si cet anticorps: - croise avec l'albumine bovine - croise avec l'albumine murine - croise avec l'albumine de singe - peut etre utilisé comme anticorps de capture avec comme Ac de detection ab19183 (HRP-conjugated anti-human serum albumin goat polyclonal Ab) Je vous remercie d'avance de votre reponse, A tres bientot,

    Read More

    Abcam community

    Verified customer

    Asked on Sep 09 2011

    Answer

    Merci de nous avoir contactés. La plupart des anti-HSA, y compris le ab19183 (conjugué HRP), ont été obtenus en utilisant la HSA entière comme immunogène. Il est donc difficile de prédire quel anti-HSA peut être utilisé en paire avec le ab19183. L'anticorps ab10241 (clone 15C7) a été utilisé en sELISA avec le ab2406 qui a été lui-même obtenu avec la HSA entière. Ceci suppose que le ab19183 peut fonctionner. Cependant, il est décrit que le monoclonal ab10241 ne croise pas avec la BSA. 2 anti-HSA me semblent intéressants : - ab84348, un polyclonal de lapin dirigé contre les acides aminés 107 à 156 de la HSA, prédit pour réagir avec l'albumine de souris. www.abcam.com/ab84348 - ab8940, qui réagit avec de nombreuses espèces, y compris la souris et la vache. www.abcam.com/ab8940 J'espère que ces informations vous aideront dans votre choix. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions et si vous désirez bénéficier de notre offre de test pour les espèces ou applications non listées sur nos fiches techniques.

    Read More

    Abcam Scientific Support

    Answered on Sep 09 2011

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