HRP Anti-Human Serum Albumin antibody (ab19183)

If you would like to coat the microtiter plate wells with your protein target, you should do that first. Adding BSA after allowing the protein target to bind the wells serves to block the wells from binding any more protein (ie. the antibodies).

Here is a link to an ELISA protocol from our protocols section which may be useful:

https://www.abcam.com/index.html?pageconfig=resource&rid=11388

We would not recommend diluting the antibodies before storing. Please follow the instructions as listed on each antibody's datasheet:

For ab19183, store the whole vial at 4C.

For ab25467 and ab31790, store at +4°C short term (1-2 weeks). Aliquot (intono smaller than 10ul)and store at -20°C long term. Avoid repeated freeze / thaw cycles.

More information can be found in our Antibody storage guide. Please note that improperly stored antibodies can void the Abpromise since we don't test our antibodies in all types of storage conditions, and therefore do not know how these will impact antibody quality.

https://www.abcam.com/index.html?pageconfig=resource&rid=10795

You can find our recommended ELISA protocols here:

https://www.abcam.com/index.html?pageconfig=resource&rid=11388

I would recommend the following antibodies for your experiments:


ab7539 - Anti-Fibrinogen (HRP)

ab19183 - Anti - Human Serum Albumin (HRP)

ab25467 - Anti -Fibronectin (HRP)


Each are conjugated to HRP and do not require a secondary antibody, however, they are all conjugated to the same enzyme and thus each one must be detected in a separate well as you would not be able to distinguish one protein from another in the same well.

The first step of the conjugation method involves the oxidation of the sugar moieties of HRP into reactive aldehyde groups. In the second step, the aldehyde groups of HRP are allowed to form irreversible covalent bonds with some of the amino groups of the antibody. Since the reaction mixture is a 1:1 ratio of the antibody to HRP, the final molar ratio calculates to 3-4 HRP molecules per antibody molecule. The conjugate is assayed functionally through direct or sandwich Elisa assay (depending on the antibody), and compared with the previous one or two lots (conjugation batches).

Merci de nous avoir contactés. La plupart des anti-HSA, y compris le ab19183 (conjugué HRP), ont été obtenus en utilisant la HSA entière comme immunogène. Il est donc difficile de prédire quel anti-HSA peut être utilisé en paire avec le ab19183. L'anticorps ab10241 (clone 15C7) a été utilisé en sELISA avec le ab2406 qui a été lui-même obtenu avec la HSA entière. Ceci suppose que le ab19183 peut fonctionner. Cependant, il est décrit que le monoclonal ab10241 ne croise pas avec la BSA. 2 anti-HSA me semblent intéressants : - ab84348, un polyclonal de lapin dirigé contre les acides aminés 107 à 156 de la HSA, prédit pour réagir avec l'albumine de souris. www.abcam.com/ab84348 - ab8940, qui réagit avec de nombreuses espèces, y compris la souris et la vache. www.abcam.com/ab8940 J'espère que ces informations vous aideront dans votre choix. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions et si vous désirez bénéficier de notre offre de test pour les espèces ou applications non listées sur nos fiches techniques.