HRP Anti-IgE antibody [4F4] (ab7294)

Submit a review Q&A (1)

Key features and details

  • HRP Mouse monoclonal [4F4] to IgE
  • Reacts with: Human
  • Conjugation: HRP
  • Isotype: IgG2a

Overview

  • Product name

    HRP Anti-IgE antibody [4F4]
    See all IgE primary antibodies

  • Description

    HRP Mouse monoclonal [4F4] to IgE

  • Host species

    Mouse

  • Conjugation

    HRP

  • Specificity

    IgE Ce3-domain specific.

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length native protein (purified) corresponding to Human IgE. IgE from myelomatous human serum.

  • General notes

    Concentration varies from lot to lot and can be provided on request.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Proclin 300
    Constituents: 0.024% Monobasic dihydrogen potassium phosphate, 0.18% Dibasic monohydrogen sodium phosphate, 0.02% Potassium chloride, 0.8% Sodium chloride
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.

  • Clonality

    Monoclonal

  • Clone number

    4F4

  • Myeloma

    Sp2/0

  • Isotype

    IgG2a

  • Research areas

Target

  • Relevance

    IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.

  • Cellular localization

    Cell Membrane

  • Database links

    • Alternative names

      • Igh2 antibody
      • IGHE antibody
      • IGHEP1 antibody
      • Immunoglobulin heavy constant epsilon antibody

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab7294? Please let us know so that we can cite the reference in this datasheet.

    ab7294 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Dear Abcam tech support,
    Please advise on the following enquiry posted by our customer:
    I'm preparing some western blot experiments using the human serum or plasma hypersensitive to the allergen of interest to a recombinant
    allergen I've made. The steps for the western blot that I have
    planned are as follows:
    (1) Run SDS-PAGE of the recombinant allergen protein
    (2) Transfer the allergen protein from the SDS-PAGE gel to a nitrocellulose membrane
    (3) Incubate membrane with human serum/plasma known to be hypersensitive to the allergen (human IgE binds to allergen on the
    membrane)
    (4) Incubate membrane with mouse/rat/rabbit anti-human IgE antibody which will bind to the human IgE
    (5) Incubate membrane with anti-mouse/rate/rabbit anti-IgG conjugated with HRP (secondary antibody)
    (6) Visualise result with ECL.
    My questions are as follows:
    (a) would the mouse monoclonal to human IgE (ab7382) be appropriate?
    If so, could you let me know the price for this antibody?
    (b) do you have a secondary antibody conjucated to HRP that I could use for step 5? If so, could you let me know the price as well?
    The reason why I'm doing steps (4) and (5) instead of just step (4) with an anti-IgE conjugated to HRP is that I've been told that as IgE is usually in very low abundance in human serum/plasma, thus I would probably be unable to detect the IgE directly without doing step (5).
    But I'm not sure if there are sensitive antibodies that could do away with step (5). I have read some papers where people have skipped step
    (5) for Western Blot. Do you have a product that is sensitive enough so that I can skip step 5 altogether? If so, could you let me have the quotation as well?
    Please advise,
    In the meantime, have a nice day!
    Kind regards,

    Read More
    Answer

    Thank you for contacting us and sorry for the delay in getting back to you.

    In answer to your questions:

    1. Yes, I believe the antibody ab7382 should be suitable for the experiment you have described, however, as we have not performed a Western blot in this way (directly binding the human IgE to the membrane), we would not know exactly how the antibody would perform. It may be that you would need to perform some optimisation of the protocol in order to obtain the best results.

    2. The goat anti-mouse IgG1 (https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-HRP-pre-adsorbed-ab98693.html) should be suitable for the experiment you are planning. Sapphire will have to inform you of what the price of this will be.

    In answer to your question of if the directly conjugated, ab7294 could be used instead of using the primary ab7382 followed by the secondary antibody, I would suggest this would be possible but as you have said the signal would be weaker, so if the target is not abundant then using a secondary antibody would be beneficial. Additionally, as we have only used ab7294 in ELISAs as yet, we would not be able to know how it would perform in a Western blot. I would therefore suggest using ab7382 with a secondary antibody may be more appropriate.

    I hope this information has been of some help. If you have any further questions, please do not hesitate to ask.

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