HRP Anti-IgG2a antibody [KT92] (ab170489)
Key features and details
- HRP Rat monoclonal [KT92] to IgG2a
- Suitable for: WB, ELISA
- Reacts with: Mouse
- Conjugation: HRP
- Isotype: IgG2c
Overview
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Product name
HRP Anti-IgG2a antibody [KT92]
See all IgG2a primary antibodies -
Description
HRP Rat monoclonal [KT92] to IgG2a -
Host species
Rat -
Conjugation
HRP -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Full length protein corresponding to Mouse IgG2a.
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Positive control
- Mouse IgG2a
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
KT92 -
Isotype
IgG2c -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
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Related Products
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Applications
Our Abpromise guarantee covers the use of ab170489 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000. Predicted molecular weight: 44 kDa. | |
ELISA | 1/2000. |
Target
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Relevance
There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). The IgG2a Ig subclass plays a critical role in the pathogenesis of humoral auto-immunity and protection against pathogens. -
Cellular localization
Secreted -
Database links
- SwissProt: P01863 Mouse
- SwissProt: P01865 Mouse
- Unigene: 342177 Mouse
- Unigene: 436014 Mouse
Images
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All lanes : HRP Anti-IgG2a antibody [KT92] (ab170489) at 1/1000 dilution
Lane 1 : Mouse IgG1
Lane 2 : Mouse IgG2a
Lane 3 : Mouse IgG2b
Lane 4 : Mouse IgG3
Lane 5 : Mouse IgM
Performed under reducing conditions.
Predicted band size: 44 kDaWestern Blotting under reducing conditions using DAB as substrate.
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ELISA showing cross-reactivity to various immunoglobulins using ab170489 at 1/2000 dilution.
References (0)
ab170489 has not yet been referenced specifically in any publications.