Key features and details
- HRP Rat monoclonal [KT92] to IgG2a
- Suitable for: WB, ELISA
- Reacts with: Mouse
- Conjugation: HRP
- Isotype: IgG2c
Product nameHRP Anti-IgG2a antibody [KT92]
See all IgG2a primary antibodies
DescriptionHRP Rat monoclonal [KT92] to IgG2a
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Mouse
Full length protein corresponding to Mouse IgG2a.
- Mouse IgG2a
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab170489 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 44 kDa.|
RelevanceThere are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). The IgG2a Ig subclass plays a critical role in the pathogenesis of humoral auto-immunity and protection against pathogens.
All lanes : HRP Anti-IgG2a antibody [KT92] (ab170489) at 1/1000 dilution
Lane 1 : Mouse IgG1
Lane 2 : Mouse IgG2a
Lane 3 : Mouse IgG2b
Lane 4 : Mouse IgG3
Lane 5 : Mouse IgM
Performed under reducing conditions.
Predicted band size: 44 kDa
Western Blotting under reducing conditions using DAB as substrate.
ELISA showing cross-reactivity to various immunoglobulins using ab170489 at 1/2000 dilution.
ab170489 has not yet been referenced specifically in any publications.