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Immunology Immunoglobulins Heavy Chain IgG
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HRP Anti-IgG2a antibody [KT92] (ab170489)

  • Datasheet
  • SDS
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Western blot - HRP Anti-IgG2a antibody [KT92] (ab170489)
  • ELISA - HRP Anti-IgG2a antibody [KT92] (ab170489)

Key features and details

  • HRP Rat monoclonal [KT92] to IgG2a
  • Suitable for: WB, ELISA
  • Reacts with: Mouse
  • Conjugation: HRP
  • Isotype: IgG2c

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Overview

  • Product name

    HRP Anti-IgG2a antibody [KT92]
    See all IgG2a primary antibodies
  • Description

    HRP Rat monoclonal [KT92] to IgG2a
  • Host species

    Rat
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Full length protein corresponding to Mouse IgG2a.

  • Positive control

    • Mouse IgG2a
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.01% Thimerosal (merthiolate)
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    KT92
  • Isotype

    IgG2c
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Immunology
    • Immune System Diseases
    • Autoimmune

Associated products

  • Alternative Versions

    • Anti-IgG2a antibody [KT92] (ab166970)
  • Recombinant Protein

    • Recombinant Mouse IgG2a protein (Fc fragment) (ab179982)
  • Related Products

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
    • 450 nm Stop Solution for TMB Substrate (ab171529)
    • 650 nm Stop Solution for TMB Substrate (ab171531)
    • Immunoassay Blocking Buffer (ab171534)
    • Immunoassay Blocking (BSA Free) (ab171535)

Applications

Our Abpromise guarantee covers the use of ab170489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 44 kDa.
ELISA 1/2000.

Target

  • Relevance

    There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). The IgG2a Ig subclass plays a critical role in the pathogenesis of humoral auto-immunity and protection against pathogens.
  • Cellular localization

    Secreted
  • Database links

    • SwissProt: P01863 Mouse
    • SwissProt: P01865 Mouse
    • Unigene: 342177 Mouse
    • Unigene: 436014 Mouse

    Images

    • Western blot - HRP Anti-IgG2a antibody [KT92] (ab170489)
      Western blot - HRP Anti-IgG2a antibody [KT92] (ab170489)
      All lanes : HRP Anti-IgG2a antibody [KT92] (ab170489) at 1/1000 dilution

      Lane 1 : Mouse IgG1
      Lane 2 : Mouse IgG2a
      Lane 3 : Mouse IgG2b
      Lane 4 : Mouse IgG3
      Lane 5 : Mouse IgM

      Performed under reducing conditions.

      Predicted band size: 44 kDa



      Western Blotting under reducing conditions using DAB as substrate.

    • ELISA - HRP Anti-IgG2a antibody [KT92] (ab170489)
      ELISA - HRP Anti-IgG2a antibody [KT92] (ab170489)

      ELISA showing cross-reactivity to various immunoglobulins using ab170489 at 1/2000 dilution.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab170489? Please let us know so that we can cite the reference in this datasheet.

    ab170489 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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