Key features and details
- HRP Rat monoclonal [KT94] to IgG3
- Suitable for: WB, ELISA
- Reacts with: Mouse
- Conjugation: HRP
- Isotype: IgG2a
Product nameHRP Anti-IgG3 antibody [KT94]
See all IgG3 primary antibodies
DescriptionHRP Rat monoclonal [KT94] to IgG3
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Mouse
Full length protein corresponding to Mouse IgG3.
- Mouse IgG3
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab170490 in the following tested applications.
|WB||1/1000. Predicted molecular weight: 44 kDa.|
RelevanceIgG3, like IgG1, activates complement and has an affinity for fc receptors; thus IgG1 and IgG3 are the more effective of the IgG subclasses. An IgG3 deficiency is associated with Wiscott-Aldrich disease, systemic lupus erythematosus, juvenile diabetes mellitus, and peridontal infections. IgG anti-viral antibodies are restricted to IgG subclasses 1 and 3, with IgG3 antibodies being the first to appear in response to infection.
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All lanes : HRP Anti-IgG3 antibody [KT94] (ab170490) at 1/1000 dilution
Lane 1 : Mouse IgG1
Lane 2 : Mouse IgG2a
Lane 3 : Mouse IgG2b
Lane 4 : Mouse IgG3
Lane 5 : Mouse IgM
Performed under reducing conditions.
Predicted band size: 44 kDa
DAB substrate used
ELISA showing cross-reactivity to various immunoglobulins using ab170490 at 1/1000 dilution.
ab170490 has not yet been referenced specifically in any publications.