HRP Anti-IL-6 antibody (ab106024)

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Western blot - HRP Anti-IL-6 antibody (ab106024)

    Key features and details

    • HRP Rabbit polyclonal to IL-6
    • Suitable for: WB
    • Reacts with: Human
    • Conjugation: HRP
    • Isotype: IgG

    Overview

    • Product name

      HRP Anti-IL-6 antibody
      See all IL-6 primary antibodies

    • Description

      HRP Rabbit polyclonal to IL-6

    • Host species

      Rabbit

    • Conjugation

      HRP

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Recombinant full length protein corresponding to Human IL-6.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab106024 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/1000 - 1/5000. Predicted molecular weight: 24 kDa. In Western blot analysis of natural cell products or Human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule.

    Target

    • Function

      Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.

    • Involvement in disease

      Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
      Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.

    • Sequence similarities

      Belongs to the IL-6 superfamily.

    • Post-translational
      modifications

      N- and O-glycosylated.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • Interleukin BSF 2 antibody
        • B cell differentiation factor antibody
        • B cell stimulatory factor 2 antibody
        • B-cell stimulatory factor 2 antibody
        • BSF 2 antibody
        • BSF-2 antibody
        • BSF2 antibody
        • CDF antibody
        • CTL differentiation factor antibody
        • Cytotoxic T cell differentiation factor antibody
        • Hepatocyte stimulating factor antibody
        • Hepatocyte stimulatory factor antibody
        • HGF antibody
        • HSF antibody
        • Hybridoma growth factor antibody
        • Hybridoma growth factor Interferon beta-2 antibody
        • Hybridoma plasmacytoma growth factor antibody
        • IFN-beta-2 antibody
        • IFNB2 antibody
        • IL 6 antibody
        • IL-6 antibody
        • IL6 antibody
        • IL6_HUMAN antibody
        • Interferon beta 2 antibody
        • Interferon beta-2 antibody
        • Interleukin 6 antibody
        • Interleukin 6 (interferon beta 2) antibody
        • Interleukin BSF 2 antibody
        • Interleukin-6 antibody
        see all

      Images

      References (0)

      Publishing research using ab106024? Please let us know so that we can cite the reference in this datasheet.

      ab106024 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Question

      I would like to perform an ELISA with IL6 specific antibodies. Since I will need to immobilize the capture antibodies myself, I need to dilute both the capture and detection antibodies. Additionally I prefer to use HRP-conjugated detection antibodies. Since the IL6 Elipair kit (ab48479) only comes with a separate HRP-Streptavidin solution, I tried to find an antibody pair and standard myself. I have selected the following antibodies/standard:

      - Anti-IL6 antibody (ab6672)
      - Anti-IL6 antibody (HRP) (ab106024)
      - IL6 standard (ab134672)

      Have these antibodies been tested to be compatible as a pair? Do you have recommended buffer solutions for:

      - Coating buffer
      - Detection buffer
      - Blocking buffer
      - Wash buffer

      I could only find a possibly suitable blocking buffer (10X Blocking Buffer (ab126587)) online. I could not find any of the other buffers sold separately.

      Read More
      Answer

      Thank you for your inquiry.

      The pair of antibodies ab6672 and ab106024 may work together in sELISA, but since we haven't tested them together unfortunately we just aren't sure. Since they are both polyclonal, they will recognize multiple epitopes, but we don't know if any of those epitopes overlap.

      We do have 2 pairs of IL6 antibodies in the catalogue that have been tested in sELISA, but the corresponding detection antibodies are biotinylated. You would have to use HRP-Strep in this case. Unfortunately we don't have an HRP-conjugated IL6 that we know works well in sELISA. It sounds like you're detecting human IL6, so the second set is probably best.

      ab9730 and ab84270
      https://www.abcam.com/il6-antibody-ab9730.html
      https://www.abcam.com/ab84270.html

      ab9324 and ab84251
      https://www.abcam.com/il6-antibody-ab9324.html
      https://www.abcam.com/ab84251.html

      The standard ab134672 should work well.

      This is our recommended sELISA protocols (with buffer recommendations).

      https://www.abcam.com/10x-phosphate-buffered-saline-ab128983.html

      We also have ELISA kits where the captture antibody is already precoated: https://www.abcam.com/il6-high-sensitivity-human-elisa-kit-ab46042.html

      I hope this information helps. Please contact us with any other questions.

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