Recombinant HRP Anti-IMP3 antibody [EPR12021] (ab208869)
![Western blot - HRP Anti-IMP3 antibody [EPR12021] (ab208869)](https://www.abcam.com/ps/products/208/ab208869/Images/ab208869-310898-anti-imp3-antibody-epr12021-hrp-western-blot.jpg "Western blot - HRP Anti-IMP3 antibody [EPR12021] (ab208869)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR12021] to IMP3
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-IMP3 antibody [EPR12021]
See all IMP3 primary antibodies -
Description
HRP Rabbit monoclonal [EPR12021] to IMP3 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment within Human IMP3. The exact sequence is proprietary.
Database link: O00425 -
Positive control
- WB: HeLa, HEK293 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12021 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab208869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 70 kDa (predicted molecular weight: 63 kDa). |
Target
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Function
RNA-binding protein that act as a regulator of mRNA translation and stability. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binds to sequences in the 3'-UTR of CD44 mRNA. -
Tissue specificity
Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary and gonocytes of testis, growing oocytes, spermatogonia and semen (at protein level). Expressed in cervix adenocarcinoma, in testicular, pancreatic and renal-cell carcinomas (at protein level). Expressed ubiquitously during fetal development at 8 and 14 weeks of gestation. Expressed in ovary, testis, brain, placenta, pancreatic cancer tissues and pancreatic cancer cell lines. -
Sequence similarities
Belongs to the RRM IMP/VICKZ family.
Contains 4 KH domains.
Contains 2 RRM (RNA recognition motif) domains. -
Domain
The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA. -
Cellular localization
Nucleus. Cytoplasm. Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Localized at the connecting piece and the tail of the spermatozoa. Colocalized with CD44 mRNA in RNP granules. - Information by UniProt
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Database links
- Entrez Gene: 10643 Human
- Entrez Gene: 140488 Mouse
- Entrez Gene: 312320 Rat
- Omim: 608259 Human
- SwissProt: O00425 Human
- SwissProt: Q9CPN8 Mouse
- Unigene: 700696 Human
- Unigene: 281018 Mouse
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Alternative names
- Cancer/testis antigen 98 antibody
- CT98 antibody
- DKFZp686F1078 antibody
see all
Images
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Western blot - HRP Anti-IMP3 antibody [EPR12021] (ab208869)All lanes : HRP Anti-IMP3 antibody [EPR12021] (ab208869) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : IGF2BP3 (IMP3) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 63 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesab208869 was shown to specifically react with IMP3 in wild-type HAP1 cells as signal was lost in IGF2BP3 (IMP3) knockout cells. Wild-type and IGF2BP3 (IMP3) knockout samples were subjected to SDS-PAGE. Ab208869 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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![Western blot - HRP Anti-IMP3 antibody [EPR12021] (ab208869)](https://www.abcam.com/ps/products/208/ab208869/Images/ab208869-265755-anti-imp3-antibody-epr12021-hrp-western-blot.jpg)
Western blot - HRP Anti-IMP3 antibody [EPR12021] (ab208869)All lanes : HRP Anti-IMP3 antibody [EPR12021] (ab208869) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab208869 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-IMP3 antibody [EPR12021] (ab208869)](https://www.abcam.com/ps/products/208/ab208869/Images/ab208869-3-benefits-of-recombinant-antibodies.png)
HRP Anti-IMP3 antibody [EPR12021] (ab208869)
References (0)
ab208869 has not yet been referenced specifically in any publications.
