HRP Anti-Insulin + Proinsulin antibody [D3E7] (ab28063)
Key features and details
- HRP Mouse monoclonal [D3E7] to Insulin + Proinsulin
- Suitable for: ELISA, IHC-Fr, IHC-P, Sandwich ELISA
- Reacts with: Mouse, Rat, Cow, Human, Pig
- Conjugation: HRP
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
HRP Anti-Insulin + Proinsulin antibody [D3E7]
See all Insulin + Proinsulin primary antibodies -
Description
HRP Mouse monoclonal [D3E7] to Insulin + Proinsulin -
Host species
Mouse -
Conjugation
HRP -
Specificity
Kd for this antibody is 6.3 x 10-8M. This antibody is specific for both insulin and proinsulin -
Tested applications
Suitable for: ELISA, IHC-Fr, IHC-P, Sandwich ELISAmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human, Pig -
Immunogen
Human insulin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.05% Proclin 300
Constituents: 0.8% Sodium chloride, 0.02% Potassium chloride, 0.18% Dibasic monohydrogen sodium phosphate, 0.024% Monobasic dihydrogen potassium phosphate -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
D3E7 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Affibody® Molecule
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Alternative Versions
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sELISA pair antibody
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28063 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | (1) |
Use at an assay dependent concentration.
|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| IHC-P |
Use at an assay dependent concentration. PubMed: 24594640
|
|
| Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [D6C4] to Insulin + Proinsulin (ab8304).
Can be used as Detection antibody with recommended pair. |
| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. PubMed: 24594640 |
|
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [D6C4] to Insulin + Proinsulin (ab8304). Can be used as Detection antibody with recommended pair. |
Target
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Relevance
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Defects in insulin are the cause of familial hyperproinsulinemia. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 280829 Cow
- Entrez Gene: 3630 Human
- Entrez Gene: 16333 Mouse
- Entrez Gene: 16334 Mouse
- Entrez Gene: 24505 Rat
- Omim: 176730 Human
- SwissProt: P01317 Cow
- SwissProt: P01308 Human
see all -
Alternative names
- ILPR antibody
- ins antibody
- Insulin antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab28063 has been referenced in 3 publications.
- Kim J et al. 1-Palmitoyl-2-Linoleoyl-3-Acetyl-rac-Glycerol Attenuates Streptozotocin-Induced Pancreatic Beta Cell Damage by Promoting Glucose Transporter 2 Endocytosis. Mol Cell Biol 39:N/A (2019). PubMed: 31481450
- Kim J et al. HX-1171 attenuates pancreatic ß-cell apoptosis and hyperglycemia-mediated oxidative stress via Nrf2 activation in streptozotocin-induced diabetic model. Oncotarget 9:24260-24271 (2018). PubMed: 29849938
- Xiao F et al. Ex vivo expanded human regulatory T cells delay islet allograft rejection via inhibiting islet-derived monocyte chemoattractant protein-1 production in CD34+ stem cells-reconstituted NOD-scid IL2r?null mice. PLoS One 9:e90387 (2014). IHC-P . PubMed: 24594640