Validated using a knockout cell lineRecombinantRabMAb

Recombinant HRP Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab209944)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab209944)
  • Western blot - HRP Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab209944)
  • HRP Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab209944)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR17338] to Integrin alpha 2 - C-terminal
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Rat, Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal
    See all Integrin alpha 2 primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR17338] to Integrin alpha 2 - C-terminal

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Integrin alpha 2 aa 1150 to the C-terminus. The exact sequence is proprietary.
    Database link: P17301

  • Positive control

    • WB: A549, T-47D, C6 and wild-type HAP1 whole cell lysates and human fetal brain tissue lysates. IHC-P: Normal human colon tissue sections.

  • General notes

    Alternative versions available:
    Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548) - Knockout validated

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab209944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 170 kDa (predicted molecular weight: 129 kDa).

Target

  • Function

    Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.

  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.

  • Domain

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • BR antibody
    • CD 49b antibody
    • CD49 antigen like family member B antibody
    • CD49 antigen-like family member B antibody
    • CD49b antibody
    • CD49b antigen antibody
    • Collagen receptor antibody
    • DX5 antibody
    • Glycoprotein Ia deficiency included antibody
    • GP Ia antibody
    • GP Ia deficiency, included antibody
    • GPIa antibody
    • HPA 5 included antibody
    • HPA5 included antibody
    • Human platelet alloantigen system 5 antibody
    • Integrin alpha 2 antibody
    • Integrin alpha II antibody
    • Integrin alpha-2 antibody
    • Integrin, alpha 2 (CD49B alpha 2 subunit of VLA 2 receptor) antibody
    • ITA2_HUMAN antibody
    • ITGA2 antibody
    • Platelet alloantigen Br(a), included antibody
    • Platelet antigen Br antibody
    • Platelet glycoprotein GPIa antibody
    • Platelet glycoprotein Ia antibody
    • Platelet glycoprotein Ia/IIa antibody
    • Platelet membrane glycoprotein Ia antibody
    • Platelet receptor for collagen, deficiency of, included antibody
    • Very late activation protein 2 receptor alpha 2 subunit antibody
    • VLA 2 alpha chain antibody
    • VLA 2 antibody
    • VLA 2 subunit alpha antibody
    • VLA-2 subunit alpha antibody
    • VLA2 antibody
    • VLA2 receptor alpha 2 subunit antibody
    • VLAA2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab209944)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab209944)

    IHC image of Integrin alpha 2 staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab209944, 1/50 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - HRP Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab209944)
    Western blot - HRP Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab209944)
    All lanes : HRP Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab209944) at 1/5000 dilution

    Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg
    Lane 2 : T-47D (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
    Lane 3 : C6 (Rat glioma cell line) Whole Cell Lysate at 10 µg
    Lane 4 : Brain (Human) Tissue Lysate - fetal normal tissue at 10 µg
    Lane 5 : Wild-type HAP1 cell lysate at 20 µg
    Lane 6 : Integrin alpha 2 knockout HAP1 cell lysate at 20 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 129 kDa
    Observed band size: 170 kDa
    why is the actual band size different from the predicted?


    Exposure time: 4 minutes


    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab209944 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • HRP Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab209944)
    HRP Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab209944)

References (0)

Publishing research using ab209944? Please let us know so that we can cite the reference in this datasheet.

ab209944 has not yet been referenced specifically in any publications.

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