RecombinantRabMAb

Recombinant HRP Anti-Integrin alpha V antibody [EPR16800] (ab205474)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Integrin alpha V antibody [EPR16800] (ab205474)
  • Western blot - HRP Anti-Integrin alpha V antibody [EPR16800] (ab205474)
  • HRP Anti-Integrin alpha V antibody [EPR16800] (ab205474)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR16800] to Integrin alpha V
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-Integrin alpha V antibody [EPR16800]
    See all Integrin alpha V primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR16800] to Integrin alpha V

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant fragment within Human Integrin alpha V aa 1-250. The exact sequence is proprietary.
    Database link: P06756

  • Positive control

    • WB: Human brain tissue lysate; IHC-P: Normal human kidney tissue sections.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab205474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000. Detects a band of approximately 135 kDa (predicted molecular weight: 116 kDa).

Target

  • Function

    The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • antigen identified by monoclonal antibody L230 antibody
      • CD 51 antibody
      • CD51 antibody
      • DKFZp686A08142 antibody
      • Integrin alpha five antibody
      • integrin alpha V beta 3 antibody
      • Integrin alpha-5 antibody
      • integrin alpha-V antibody
      • Integrin alpha-V light chain antibody
      • integrin alphaVbeta3 antibody
      • integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) antibody
      • ITAV_HUMAN antibody
      • ITGAV antibody
      • MSK 8 antibody
      • Msk8 antibody
      • Vitronectin receptor subunit alpha antibody
      • VNRA antibody
      • VTNR antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Integrin alpha V antibody [EPR16800] (ab205474)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Integrin alpha V antibody [EPR16800] (ab205474)

      IHC image of Integrin alpha V staining in a section of formalin-fixed paraffin-embedded normal human kidney*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab205474, 1/2500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Western blot - HRP Anti-Integrin alpha V antibody [EPR16800] (ab205474)
      Western blot - HRP Anti-Integrin alpha V antibody [EPR16800] (ab205474)
      HRP Anti-Integrin alpha V antibody [EPR16800] (ab205474) at 1/2000 dilution + Human brain lysate at 10 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 116 kDa
      Observed band size: 135 kDa
      why is the actual band size different from the predicted?


      Exposure time: 2 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205474 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • HRP Anti-Integrin alpha V antibody [EPR16800] (ab205474)
      HRP Anti-Integrin alpha V antibody [EPR16800] (ab205474)

    References (0)

    Publishing research using ab205474? Please let us know so that we can cite the reference in this datasheet.

    ab205474 has not yet been referenced specifically in any publications.

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