HRP Anti-Kappa light chain antibody (ab46527)

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Key features and details

  • HRP Goat polyclonal to Kappa light chain
  • Reacts with: Mouse
  • Conjugation: HRP
  • Isotype: IgG

Overview

  • Product name

    HRP Anti-Kappa light chain antibody
    See all Kappa light chain primary antibodies

  • Description

    HRP Goat polyclonal to Kappa light chain

  • Host species

    Goat

  • Conjugation

    HRP

  • Specificity

    This antibody recognises mouse kappa light chains; both bound and free.

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Mouse paraproteins with kappa light chains

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituents: PBS, 50% Glycerol
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    This antibody was made from pooled antisera from goats immunised with mouse paraproteins bearing kappa light chains. Antiserum was purified by affinity chromatography on pooled mouse Igs with kappa light chains. The antiserum was cross adsorbed on pooled mouse Igs with lambda light chains.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Target

  • Relevance

    Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.

  • Cellular localization

    Cytoplasmic and Secreted

  • Database links

    • Alternative names

      • HCAK 1 antibody
      • HCAK1 antibody
      • Ig kappa chain C region antibody
      • IGKC antibody
      • IGKCD antibody
      • Immunoglobulin InV antibody
      • Immunoglobulin kappa constant antibody
      • Immunoglobulin kappa constant region antibody
      • Immunoglobulin kappa light chain antibody
      • Immunoglobulin kappa light chain constant region antibody
      • Immunoglobulin KM antibody
      • Kappa 1 immunoglobulin light chain antibody
      • kappa light chain antibody
      • Km antibody
      • MGC111575 antibody
      • MGC62011 antibody
      • MGC72072 antibody
      • MGC88770 antibody
      • MGC88771 antibody
      • MGC88809 antibody
      see all

    References (0)

    Publishing research using ab46527? Please let us know so that we can cite the reference in this datasheet.

    ab46527 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

    Dear xxxxx,


    Thanks for your email.

    In mice, 95% of the light chains are κ and only 5% are λ, perhaps you can quote both κ light chain and λ light chain.

    Thanks and best regards,

    Read More
    Answer

    Thank you for your reply.

    I have had a look at the antibodies we have available form our catalogue and have come up with the following for you to consider:

    against kappa light chain:

    Rat monoclonal ab11662

    Rat monoclonal ab99614

    Rabbit polyclonal ab103827

    against kappa light chain, HRP conjugated:

    Goat polyclonal ab46527

    Rat monoclonal ab99617

    against lambda light chain:

    Rat monoclonal ab99622

    against lambda light chain HRP conjugated

    Rat monoclonal ab99625

    I hope this information has been of help. If you have any further questions, please do not hesitate to contact me again.

    Read More

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