HRP Anti-Kappa light chain antibody [KT102] (ab170326)
![Western blot - HRP Anti-Kappa light chain antibody [KT102] (ab170326)](https://www.abcam.com/ps/products/170/ab170326/Images/ab170326-169469-anti-kappa-light-chain-antibody-kt102-hrp-western-blot.jpg "Western blot - HRP Anti-Kappa light chain antibody [KT102] (ab170326)")
Key features and details
- HRP Mouse monoclonal [KT102] to Kappa light chain
- Suitable for: WB, ELISA
- Reacts with: Rat
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-Kappa light chain antibody [KT102]
See all Kappa light chain primary antibodies -
Description
HRP Mouse monoclonal [KT102] to Kappa light chain -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Rat
Does not react with: Mouse, Rabbit, Goat, Human -
Immunogen
Synthetic peptide corresponding to Rat Kappa light chain.
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Positive control
- Rat Ig Kappa light chain
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General notes
ab170326 can be used for rat IgG isotyping.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab170326 is purified through a Protein A column and labeled with horseradish peroxidase. -
Clonality
Monoclonal -
Clone number
KT102 -
Isotype
IgG1 -
Research areas
Associated products
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Related Products
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
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- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
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- Immunoassay Blocking (BSA Free) (ab171535)
Applications
Our Abpromise guarantee covers the use of ab170326 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. This dilution works using DAB as substrate. More dilution is needed for ECL system. |
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| ELISA | 1/2000. |
Target
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Relevance
Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83. -
Cellular localization
Cytoplasmic and Secreted -
Database links
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Alternative names
- HCAK 1 antibody
- HCAK1 antibody
- Ig kappa chain C region antibody
see all
Images
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Western blot - HRP Anti-Kappa light chain antibody [KT102] (ab170326)All lanes : HRP Anti-Kappa light chain antibody [KT102] (ab170326) at 1/1000 dilution
Lane 1 : rat IgM
Lane 2 : rat IgG2c
Lane 3 : rat IgG2b
Lane 4 : rat IgG2a
Lane 5 : rat IgG1
Performed under reducing conditions. -
![ELISA - HRP Anti-Kappa light chain antibody [KT102] (ab170326)](https://www.abcam.com/ps/products/170/ab170326/Images/ab170326-169468-anti-kappa-light-chain-antibody-kt102-hrp-elisa.jpg)
ELISA - HRP Anti-Kappa light chain antibody [KT102] (ab170326)ELISA for crossreactivity to various immunoglobulins.
References (0)
ab170326 has not yet been referenced specifically in any publications.
