HRP Anti-KDEL Receptor antibody [KR-10] (ab183379)
![Western blot - HRP Anti-KDEL Receptor antibody [KR-10] (ab183379)](https://www.abcam.com/ps/products/183/ab183379/Images/ab183379-310930-183379444.png "Western blot - HRP Anti-KDEL Receptor antibody [KR-10] (ab183379)")
Key features and details
- HRP Mouse monoclonal [KR-10] to KDEL Receptor
- Suitable for: WB
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-KDEL Receptor antibody [KR-10]
See all KDEL Receptor primary antibodies -
Description
HRP Mouse monoclonal [KR-10] to KDEL Receptor -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Predicted to work with: Mouse, Rat, Cow, Human, Xenopus laevis, Xenopus tropicalis
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Immunogen
Synthetic peptide corresponding to Cow KDEL Receptor aa 192-212 conjugated to keyhole limpet haemocyanin. 21 residue synthetic peptide.
Sequence:CDFFYLYITKVLKGKKLSLPA
Database link: P33946 -
Positive control
- WB: Rat tissue lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
KR-10 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
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Related Products
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- Anti-KDEL Receptor antibody [KR-10] (ab69659)
Applications
Our Abpromise guarantee covers the use of ab183379 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). |
Target
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Relevance
Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, which is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. The protein encoded by this gene was the first member of the family to be identified, and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product. -
Cellular localization
Endoplasmic reticulum -
Database links
- Entrez Gene: 618184 Cow
- Entrez Gene: 10945 Human
- Entrez Gene: 68137 Mouse
- Entrez Gene: 361577 Rat
- Omim: 131235 Human
- SwissProt: P33946 Cow
- SwissProt: P24390 Human
- SwissProt: Q99JH8 Mouse
see all -
Alternative names
- ER lumen protein retaining receptor 1 antibody
- ERD 2 antibody
- ERD2 antibody
see all
Images
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Western blot - HRP Anti-KDEL Receptor antibody [KR-10] (ab183379)Unconjugated version of ab183379, 2 hours at RT at 1/1000 dilution + Rat tissue lysate at 15 µg
Secondary
Sheep Anti-Mouse IgG: HRP, 1 hour at RT
Predicted band size: 25 kDaBlock: 1.5% BSA for 30 minutes at RT.
This data was produced using the unconjugated version of this antibody.
Protocols
Datasheets and documents
References (0)
ab183379 has not yet been referenced specifically in any publications.
