HRP Anti-KDEL Receptor antibody [KR-10] (ab183379)
Key features and details
- HRP Mouse monoclonal [KR-10] to KDEL Receptor
- Suitable for: WB
- Reacts with: Rat
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-KDEL Receptor antibody [KR-10]
See all KDEL Receptor primary antibodies -
Description
HRP Mouse monoclonal [KR-10] to KDEL Receptor -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
Application Species WB Rat -
Immunogen
Synthetic peptide corresponding to Cow KDEL Receptor aa 150 to the C-terminus conjugated to keyhole limpet haemocyanin. 21 residue synthetic peptide.
Database link: P33946 -
Positive control
- WB: Rat tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
KR-10 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
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Related Products
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
- Anti-KDEL Receptor antibody [KR-10] (ab69659)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183379 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
WB |
Rat
|
All applications |
Mouse
Cow
Human
Xenopus laevis
Xenopus tropicalis
|
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). |
Target
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Relevance
Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, which is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. The protein encoded by this gene was the first member of the family to be identified, and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product. -
Cellular localization
Endoplasmic reticulum -
Database links
- Entrez Gene: 618184 Cow
- Entrez Gene: 10945 Human
- Entrez Gene: 68137 Mouse
- Entrez Gene: 361577 Rat
- Omim: 131235 Human
- SwissProt: P33946 Cow
- SwissProt: P24390 Human
- SwissProt: Q99JH8 Mouse
see all -
Alternative names
- ER lumen protein retaining receptor 1 antibody
- ERD 2 antibody
- ERD2 antibody
see all
Images
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Unconjugated version of ab183379, 2 hours at RT at 1/1000 dilution + Rat tissue lysate at 15 µg
Secondary
Sheep Anti-Mouse IgG: HRP, 1 hour at RT
Predicted band size: 25 kDaBlock: 1.5% BSA for 30 minutes at RT.
This data was produced using the unconjugated version of this antibody.
Datasheets and documents
References (0)
ab183379 has not yet been referenced specifically in any publications.