Validated using a knockout cell lineRecombinantRabMAb

Recombinant HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)

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Western blot - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)
  • Western blot - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)
  • HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR6825] to KDM1/LSD1 - Nuclear Marker
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Rat, Human
  • Conjugation: HRP

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free Unconjugated

Overview

  • Product name

    HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker
    See all KDM1/LSD1 primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR6825] to KDM1/LSD1 - Nuclear Marker

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab166919)

  • Positive control

    • WB: HeLa, HAP1, Jurkat and PC12 whole cell lysates. IHC-P: FFPE Human Testis Normal

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin 300 Solution
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR6825

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab195897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 92 kDa).Can be blocked with KDM1/LSD1 peptide (ab166919).
Notes
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 92 kDa).Can be blocked with KDM1/LSD1 peptide (ab166919).

Target

  • Function

    Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.

  • Tissue specificity

    Ubiquitously expressed.

  • Sequence similarities

    Belongs to the flavin monoamine oxidase family.
    Contains 1 SWIRM domain.

  • Domain

    The SWIRM domain may act as an anchor site for a histone tail.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Amine oxidase (flavin containing) domain 2 antibody
      • Amine oxidase, flavin containing, 2 antibody
      • AOF2 antibody
      • BHC110 antibody
      • BRAF35 HDAC complex protein BHC110 antibody
      • BRAF35-HDAC complex protein BHC110 antibody
      • BRAF35/HDAC complex, 110-kD subunit antibody
      • CPRF antibody
      • EC1 antibody
      • FAD binding protein BRAF35 HDAC complex, 110 kDa subunit antibody
      • Flavin-containing amine oxidase domain-containing protein 2 antibody
      • KDM 1 antibody
      • KDM1 antibody
      • Kdm1a antibody
      • KDM1A_HUMAN antibody
      • KIAA0601 antibody
      • LSD 1 antibody
      • LSD1 antibody
      • Lysine (K) specific demethylase 1 antibody
      • Lysine (K) specific demethylase 1A antibody
      • Lysine demethylase 1A antibody
      • Lysine specific histone demethylase 1 antibody
      • Lysine specific histone demethylase 1A antibody
      • Lysine-specific demethylase 1 antibody
      • Lysine-specific demethylase 1A antibody
      • Lysine-specific histone demethylase 1A antibody
      see all

    Images

    • Western blot - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)
      Western blot - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)
      All lanes : HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897) at 1/5000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : KDM1A (KDM1 / LSD1) knockout HAP1 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 92 kDa
      Observed band size: 110 kDa why is the actual band size different from the predicted?


      Exposure time: 20 minutes


      ab195897 was shown to specifically react with KDM1 / LSD1 in wild-type HAP1 cells as signal was lost in KDM1A (KDM1 / LSD1) knockout cells. Wild-type and KDM1A (KDM1 / LSD1) knockout samples were subjected to SDS-PAGE. Ab195897 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)

      IHC image of KDM1/LSD1 staining in a section of formalin-fixed paraffin-embedded normal human testis*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab195897 at 1/50 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody. 

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. 

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Western blot - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)
      Western blot - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)
      All lanes : HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897) at 1/5000 dilution

      Lane 1 : HeLa whole cell lysate (ab150035)
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 92 kDa
      Observed band size: 110 kDa why is the actual band size different from the predicted?


      Exposure time: 4 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab195897 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)
      HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)

    References (0)

    Publishing research using ab195897? Please let us know so that we can cite the reference in this datasheet.

    ab195897 has not yet been referenced specifically in any publications.

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