Recombinant HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)
![Western blot - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)](https://www.abcam.com/ps/products/195/ab195897/Images/ab195897-310964-anti-kdm1-lsd1-antibody-epr6825-nuclear-marker-hrp-western-blot.jpg "Western blot - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR6825] to KDM1/LSD1 - Nuclear Marker
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Rat, Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker
See all KDM1/LSD1 primary antibodies -
Description
HRP Rabbit monoclonal [EPR6825] to KDM1/LSD1 - Nuclear Marker -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide within Human KDM1/ LSD1 aa 50-150. The exact sequence is proprietary.
(Peptide available asab166919) -
Positive control
- WB: HeLa, HAP1, Jurkat and PC12 whole cell lysates. IHC-P: FFPE Human Testis Normal
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6825 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker and ChIP Grade (ab129195)
- Alexa Fluor® 488 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab184811)
- Alexa Fluor® 647 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195305)
- Anti-KDM1/LSD1 antibody [EPR6825] - BSA and Azide free (ab224270)
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab195897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 92 kDa).Can be blocked with KDM1 / LSD1 peptide (ab166919). |
Target
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Function
Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the flavin monoamine oxidase family.
Contains 1 SWIRM domain. -
Domain
The SWIRM domain may act as an anchor site for a histone tail. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 23028 Human
- Entrez Gene: 99982 Mouse
- Entrez Gene: 500569 Rat
- Omim: 609132 Human
- SwissProt: O60341 Human
- SwissProt: Q6ZQ88 Mouse
- Unigene: 591518 Human
- Unigene: 28540 Mouse
see all -
Alternative names
- Amine oxidase (flavin containing) domain 2 antibody
- Amine oxidase, flavin containing, 2 antibody
- AOF2 antibody
see all
Images
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Western blot - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)All lanes : HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : KDM1A (KDM1 / LSD1) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 92 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesab195897 was shown to specifically react with KDM1 / LSD1 in wild-type HAP1 cells as signal was lost in KDM1A (KDM1 / LSD1) knockout cells. Wild-type and KDM1A (KDM1 / LSD1) knockout samples were subjected to SDS-PAGE. Ab195897 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)](https://www.abcam.com/ps/products/195/ab195897/Images/ab195897-256011-anti-kdm1-lsd1-antibody-epr6825-nuclear-marker-hrp-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)IHC image of KDM1/LSD1 staining in a section of formalin-fixed paraffin-embedded normal human testis*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab195897 at 1/50 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)](https://www.abcam.com/ps/products/195/ab195897/Images/ab195897-241887-anti-kdm1-lsd1-antibody-epr6825-nuclear-marker-hrp-western-blot.jpg)
Western blot - HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)All lanes : HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897) at 1/5000 dilution
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab195897 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)](https://www.abcam.com/ps/products/195/ab195897/Images/ab195897-4-benefits-of-recombinant-antibodies.png)
HRP Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker (ab195897)
Protocols
References (0)
ab195897 has not yet been referenced specifically in any publications.
