RecombinantRabMAb

Recombinant HRP Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab208366)

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Western blot - HRP Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab208366)
  • HRP Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab208366)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EP1565Y] to Lactate Dehydrogenase B/LDH-B
  • Suitable for: WB
  • Reacts with: Human
  • Conjugation: HRP

Conjugates logo Related conjugates and formulations

Carrier Free Unconjugated

Overview

  • Product name

    HRP Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y]
    See all Lactate Dehydrogenase B/LDH-B primary antibodies

  • Description

    HRP Rabbit monoclonal [EP1565Y] to Lactate Dehydrogenase B/LDH-B

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa whole cell lysate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab208366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).
Notes
WB
1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).

Target

  • Pathway

    Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.

  • Involvement in disease

    Note=Defects in LDHB result in deficiency of lactate dehydrogenase, a condition with no clear symptomatic consequences. Lactate dehydrogenase deficiency can probably be considered a non-disease.

  • Sequence similarities

    Belongs to the LDH/MDH superfamily. LDH family.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • Epididymis secretory protein Li 281 antibody
      • HEL S 281 antibody
      • L lactate dehydrogenase B chain antibody
      • L-lactate dehydrogenase B chain antibody
      • Lactate Dehydrogenase B antibody
      • Lactate dehydrogenase H chain antibody
      • LDH B antibody
      • LDH H antibody
      • LDH heart subunit antibody
      • LDH-B antibody
      • LDH-H antibody
      • LDHB antibody
      • LDHB_HUMAN antibody
      • LDHBD antibody
      • LDHH antibody
      • Renal carcinoma antigen NY REN 46 antibody
      • Renal carcinoma antigen NY-REN-46 antibody
      • TRG-5 antibody
      • TRG5 antibody
      see all

    Images

    • Western blot - HRP Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab208366)
      Western blot - HRP Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab208366)
      HRP Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab208366) at 1/5000 dilution + HeLa whole cell lysate (ab150035) at 10 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 37 kDa
      Observed band size: 35 kDa why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab208366 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • HRP Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab208366)
      HRP Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab208366)

    References (1)

    Publishing research using ab208366? Please let us know so that we can cite the reference in this datasheet.

    ab208366 has been referenced in 1 publication.

    • Smith H  et al. The utilisation of glutamine and glucose by a 3-D tumour model trapped in quiescence. Int J Biochem Cell Biol 133:105935 (2021). PubMed: 33529714

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