Recombinant HRP Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab208366)
![Western blot - HRP Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab208366)](https://www.abcam.com/ps/products/208/ab208366/Images/ab208366-270495-anti-lactate-dehydrogenase-b-ldh-b-antibody-ep1565y-hrp-western-blot.jpg "Western blot - HRP Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab208366)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP1565Y] to Lactate Dehydrogenase B/LDH-B
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y]
See all Lactate Dehydrogenase B/LDH-B primary antibodies -
Description
HRP Rabbit monoclonal [EP1565Y] to Lactate Dehydrogenase B/LDH-B -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Lactate Dehydrogenase B/LDH-B aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P07195 -
Positive control
- WB: HeLa whole cell lysate.
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General notes
Previously labelled as Lactate Dehydrogenase B.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EP1565Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab208366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa). |
Target
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Pathway
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. -
Involvement in disease
Note=Defects in LDHB result in deficiency of lactate dehydrogenase, a condition with no clear symptomatic consequences. Lactate dehydrogenase deficiency can probably be considered a non-disease. -
Sequence similarities
Belongs to the LDH/MDH superfamily. LDH family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 3945 Human
- Omim: 150100 Human
- SwissProt: P07195 Human
- Unigene: 446149 Human
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Alternative names
- Epididymis secretory protein Li 281 antibody
- HEL S 281 antibody
- L lactate dehydrogenase B chain antibody
see all
Images
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Western blot - HRP Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab208366)HRP Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab208366) at 1/5000 dilution +
HeLa whole cell lysate (ab150035) at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab208366 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab208366)](https://www.abcam.com/ps/products/208/ab208366/Images/ab208366-2-benefits-of-recombinant-antibodies.png)
HRP Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab208366)
References (0)
ab208366 has not yet been referenced specifically in any publications.
