RecombinantRabMAb

Recombinant HRP Anti-LDL Receptor antibody [EP1553Y] (ab195516)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-LDL Receptor antibody [EP1553Y] (ab195516)
  • HRP Anti-LDL Receptor antibody [EP1553Y] (ab195516)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EP1553Y] to LDL Receptor
  • Suitable for: IHC-P
  • Reacts with: Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-LDL Receptor antibody [EP1553Y]
    See all LDL Receptor primary antibodies

  • Description

    HRP Rabbit monoclonal [EP1553Y] to LDL Receptor

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide within Human LDL Receptor (C terminal). The exact sequence is proprietary.

  • Positive control

    • IHC/P: Normal human adrenal gland tissue.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab195516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.

  • Involvement in disease

    Defects in LDLR are the cause of familial hypercholesterolemia (FH) [MIM:143890]; a common autosomal semi-dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis).

  • Sequence similarities

    Belongs to the LDLR family.
    Contains 3 EGF-like domains.
    Contains 7 LDL-receptor class A domains.
    Contains 6 LDL-receptor class B repeats.

  • Post-translational
    modifications

    N- and O-glycosylated.
    Ubiquitinated by MYLIP leading to degradation.

  • Cellular localization

    Cell membrane. Endomembrane system. Membrane > clathrin-coated pit. Found distributed from the plasma membrane to intracellular compartments.
  • Information by UniProt

  • Database links

    • Alternative names

      • FH antibody
      • FHC antibody
      • LDL R antibody
      • LDL receptor antibody
      • LDLCQ2 antibody
      • Ldlr antibody
      • LDLR_HUMAN antibody
      • Low Density Lipoprotein Receptor antibody
      • Low density lipoprotein receptor class A domain containing protein 3 antibody
      • Low density lipoprotein receptor familial hypercholesterolemia antibody
      • Low-density lipoprotein receptor antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-LDL Receptor antibody [EP1553Y] (ab195516)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-LDL Receptor antibody [EP1553Y] (ab195516)

      IHC image of LDL Receptor staining in a section of formalin-fixed paraffin-embedded normal human adrenal gland, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab195516 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • HRP Anti-LDL Receptor antibody [EP1553Y] (ab195516)
      HRP Anti-LDL Receptor antibody [EP1553Y] (ab195516)

    References (0)

    Publishing research using ab195516? Please let us know so that we can cite the reference in this datasheet.

    ab195516 has not yet been referenced specifically in any publications.

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