RecombinantRabMAb

Recombinant HRP Anti-LEF1 antibody [EPR2029Y] (ab197623)

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Western blot - HRP Anti-LEF1 antibody [EPR2029Y] (ab197623)
  • HRP Anti-LEF1 antibody [EPR2029Y] (ab197623)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR2029Y] to LEF1
  • Suitable for: WB
  • Reacts with: Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-LEF1 antibody [EPR2029Y]
    See all LEF1 primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR2029Y] to LEF1

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat whole cell lysate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab197623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/5000. Detects a band of approximately 44,55,70 kDa (predicted molecular weight: 44 kDa).
Notes
WB
1/5000. Detects a band of approximately 44,55,70 kDa (predicted molecular weight: 44 kDa).

Target

  • Function

    Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.

  • Tissue specificity

    Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.

  • Sequence similarities

    Belongs to the TCF/LEF family.
    Contains 1 HMG box DNA-binding domain.

  • Domain

    Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.

  • Cellular localization

    Nucleus. Found in nuclear bodies upon PIASG binding.
  • Information by UniProt

  • Database links

    • Alternative names

      • DKFZp586H0919 antibody
      • FLJ46390 antibody
      • LEF 1 antibody
      • LEF-1 antibody
      • Lef1 antibody
      • LEF1_HUMAN antibody
      • Lymphoid enhancer binding factor 1 antibody
      • Lymphoid enhancer-binding factor 1 antibody
      • T cell specific transcription factor 1 alpha antibody
      • T cell-specific transcription factor 1-alpha antibody
      • TCF 1 alpha antibody
      • TCF1 alpha antibody
      • TCF1-alpha antibody
      • TCF10 antibody
      • TCF1alpha antibody
      • TCF7L3 antibody
      • Transcription factor T cell specific 1 alpha antibody
      see all

    Images

    • Western blot - HRP Anti-LEF1 antibody [EPR2029Y] (ab197623)
      Western blot - HRP Anti-LEF1 antibody [EPR2029Y] (ab197623)
      HRP Anti-LEF1 antibody [EPR2029Y] (ab197623) at 1/5000 dilution + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 44 kDa
      Observed band size: 44,55,70 kDa why is the actual band size different from the predicted?


      Exposure time: 8 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab197623 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

       

    • HRP Anti-LEF1 antibody [EPR2029Y] (ab197623)
      HRP Anti-LEF1 antibody [EPR2029Y] (ab197623)

    References (0)

    Publishing research using ab197623? Please let us know so that we can cite the reference in this datasheet.

    ab197623 has not yet been referenced specifically in any publications.

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