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I would like to know if the antibody ab20561 (HRP) is the same than the ab20527. Do you know if this both antibodies reacts against the same epitops??
Asked on Sep 19 2013
ab20561 and ab20527 are produced in the same lab and in exactly the same way, using a whole cell preparation of Legionella pneumophila; ATCC #33152 to immunise the rabbits. They are therefore the same antibody but conjugated to different molecules. ab20561 is HRP conjugated whereas ab20527 is biotin conjugated.
Since these antibodies are polyclonal antibodies, they will recognise multiple epitopes on any one antigen which is different to monoclonal antibodies which detect only one epitope on the antigen. Therefore, the epitopes recognised by these antibodies will be batch dependent and so we cannot say whether these two antibodies will react against the same epitope.
Elisa ThomasAbcam Scientific Support
Answered on Sep 19 2013