RecombinantRabMAb

Recombinant HRP Anti-Liver Arginase antibody [EPR6672(B)] (ab195510)

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Western blot - HRP Anti-Liver Arginase antibody [EPR6672(B)] (ab195510)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Liver Arginase antibody [EPR6672(B)] (ab195510)
  • HRP Anti-Liver Arginase antibody [EPR6672(B)] (ab195510)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR6672(B)] to Liver Arginase
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-Liver Arginase antibody [EPR6672(B)]
    See all Liver Arginase primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR6672(B)] to Liver Arginase

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Liver Arginase aa 300 to the C-terminus.

  • Positive control

    • WB: Human Fetal Liver tissue lysate. IHC-P: normal human liver tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab195510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Pathway

    Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.

  • Involvement in disease

    Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia.

  • Sequence similarities

    Belongs to the arginase family.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • A I antibody
      • Al antibody
      • ARG 1 antibody
      • arg1 antibody
      • ARGI1_HUMAN antibody
      • Arginase 1 antibody
      • Arginase liver antibody
      • Arginase type I antibody
      • Arginase, liver antibody
      • Arginase-1 antibody
      • Arginase1 antibody
      • Liver type arginase antibody
      • Liver-type arginase antibody
      • Type I arginase antibody
      see all

    Images

    • Western blot - HRP Anti-Liver Arginase antibody [EPR6672(B)] (ab195510)
      Western blot - HRP Anti-Liver Arginase antibody [EPR6672(B)] (ab195510)
      HRP Anti-Liver Arginase antibody [EPR6672(B)] (ab195510) at 1/2000 dilution + Liver (Human) Whole Cell Lysate - fetal normal tissue at 10 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 35 kDa
      Observed band size: 35 kDa


      Exposure time: 10 seconds


      This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab195510 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Liver Arginase antibody [EPR6672(B)] (ab195510)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Liver Arginase antibody [EPR6672(B)] (ab195510)

      IHC image of Liver Arginase staining in a section of formalin-fixed paraffin-embedded normal human liver tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab195510 at 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • HRP Anti-Liver Arginase antibody [EPR6672(B)] (ab195510)
      HRP Anti-Liver Arginase antibody [EPR6672(B)] (ab195510)

    References (0)

    Publishing research using ab195510? Please let us know so that we can cite the reference in this datasheet.

    ab195510 has not yet been referenced specifically in any publications.

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